Artículo
Oestrogen, Progesterone and Oxytocin Receptors and COX-2 Expression in Endometrial Biopsy Samples from the Induction of Ovulation to Luteolysis in Llamas (Lama glama)
Bianchi, Carolina Paula
; Meikle, A.; Alvarez, M.A.; Benavente, Micaela Andrea
; Cavilla, María Verónica; Rodriguez, E.; Aba, Marcelo Alfredo
Fecha de publicación:
02/2013
Editorial:
Wiley-blackwell Publishing, Inc
Revista:
Reproduction In Domestic Animals (1990)
ISSN:
0936-6768
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Endometrial expression of oestrogen (ERa), progesterone (PR) and oxytocin receptor (OR) and cyclooxygenase-2 (COX-2) was evaluated from the induction of ovulation to luteolysis in llamas. Ovarian activity was daily assessed by ultrasonography in five females. Ovulation was induced immediately after the detection of an ovulatory follicle by a GnRH injection (Day 0). Endometrial samples were obtained by transcervical biopsies from the left and right horns on day 0 and days 4, 8, 10 and 12 post-GnRH. Blood samples were collected daily for progesterone and estradiol-17b determinations by RIA. An immunohistochemical technique was used to study receptors population and COX-2 expression which were then evaluated by two independent observers. The expression of ERa and PR was highest on day 0 in the luminal epithelium and stroma in association with high plasma estradiol-17b concentrations. Thereafter, a decrease in ERa population was registered on day 4 and a new increase of its expression was observed between days 8 and 12 in those cell types. Conversely, PR population was gradually down-regulated until its lowest expression was reached on day 10 post-GnRH in the luminal epithelium. Content of OR was similar throughout the study in all cell types. The expression of COX-2 was highest from day 8 to 12 post-GnRH in the luminal epithelium, in relation to the time of maximal PGF2a release. Both steroid receptors populations and COX-2 expression were similar between horns. Meanwhile, OR expression was higher in the right than in the left uterine horn. In summary, this study showed that the loss of endometrium sensitivity to progesterone by days 8?10 post-induction of ovulation and the concomitant increase of COX-2 expression could play a key role in the mechanism of luteolysis and somehow be related to the short corpus luteum lifespan of llamas.
Palabras clave:
Hormone Receptors
,
Endometrium
,
Luteolysis
,
Llamas
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - TANDIL)
Articulos de CTRO CIENTIFICO TECNOLOGICO CONICET - TANDIL
Articulos de CTRO CIENTIFICO TECNOLOGICO CONICET - TANDIL
Articulos(CIVETAN)
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Citación
Bianchi, Carolina Paula; Meikle, A.; Alvarez, M.A.; Benavente, Micaela Andrea; Cavilla, María Verónica; et al.; Oestrogen, Progesterone and Oxytocin Receptors and COX-2 Expression in Endometrial Biopsy Samples from the Induction of Ovulation to Luteolysis in Llamas (Lama glama); Wiley-blackwell Publishing, Inc; Reproduction In Domestic Animals (1990); 48; 2-2013; 681-690
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