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dc.contributor.author
Rodríguez, Francisco Isabelino
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dc.contributor.author
Procura, Francisco
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dc.contributor.author
Bueno, Dante Javier
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dc.date.available
2020-03-06T20:41:28Z
dc.date.issued
2018-06
dc.identifier.citation
Rodríguez, Francisco Isabelino; Procura, Francisco; Bueno, Dante Javier; Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces; Poultry Science Association; Poultry Science; 97; 11; 6-2018; 3826-3836
dc.identifier.issn
0032-5791
dc.identifier.uri
http://hdl.handle.net/11336/98994
dc.description.abstract
The present work compared 7 different culture methods and 3 selective-differential plating media for Salmonella ser. Enteritidis (SE) and S. Ser. Typhimurium (ST) isolation using artificially contaminated poultry feces. The sensitivity (Se) and accuracy (AC) values increased when Modified Semisolid Rappaport Vassiliadis (MSRV) methods were used in place of the Tetrathionate (TT) or Tetrathionate Hajna broth (TTH) method in the enrichment step. However, there was no significant difference between the pre-enrichment incubation at 4 to 6 and 18 to 24 h for MSRV5 and MSRV24 methods, respectively. All Salmonella strains were recovered in the lowest dilutions tested for MSRV24 and 3 out of 4 for MSRV5 methods (2 to 10 cfu/25 g). The TT and TTH methods showed a detection limit between 2.2 × 101 and 1.0 × 106 cfu/25 g of fecal sample. The agreement was variable between the methods. However, there was a very good agreement between the MSRV5 and MSRV24 methods, and between tetrathionate direct (TTD, no pre-enrichment media used) and buffered peptone water 18 to 24 h Tetrathionate broth combination (TT24 method) for Salmonella strains. The 3 selective-differential plating media showed an agreement between fair and excellent. They performed a high Se and AC in the MSRV methods for Salmonella strains. There was a significant difference between center and periphery for MSRV methods, and there was a fair agreement between them for all strains. The MSRV methods are better than TT/TTH methods for the isolation of different strains of SE and ST in poultry fecal samples. The MSRV5 method can be used to reduce the time for the detection of SE and ST in these samples. Furthermore, a loopful of the periphery of the growth should be streaked onto differential-selective plating media, even in the absence of halo, to decrease the number of false negative results.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Poultry Science Association
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
CULTURE METHOD
dc.subject
POULTRY FECES
dc.subject
SALMONELLA
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Comparison of 7 culture methods for Salmonella serovar Enteritidis and Salmonella serovar Typhimurium isolation in poultry feces
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-03-04T15:42:45Z
dc.journal.volume
97
dc.journal.number
11
dc.journal.pagination
3826-3836
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Rodríguez, Francisco Isabelino. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Parana. Grupo D/genética, Mejoram. y Biotecnología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
dc.description.fil
Fil: Procura, Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Parana. Grupo D/genética, Mejoram. y Biotecnología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Bueno, Dante Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Parana. Grupo D/genética, Mejoram. y Biotecnología Vegetal; Argentina. Universidad Autónoma de Entre Ríos; Argentina
dc.journal.title
Poultry Science
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3382/ps/pey259
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0032579119304869
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