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dc.contributor.author
Ramírez Tapias, Yuly Andrea
dc.contributor.author
Rivero, Cintia Wanda
dc.contributor.author
Giraldo Estrada, Catalina
dc.contributor.author
Britos, Claudia Noelia
dc.contributor.author
Trelles, Jorge Abel
dc.date.available
2020-03-05T18:23:30Z
dc.date.issued
2018-09
dc.identifier.citation
Ramírez Tapias, Yuly Andrea; Rivero, Cintia Wanda; Giraldo Estrada, Catalina; Britos, Claudia Noelia; Trelles, Jorge Abel; Biodegradation of vegetable residues by polygalacturonase-agar using a trickle-bed bioreactor; Institution of Chemical Engineers; Food and Bioproducts Processing; 111; 9-2018; 54-61
dc.identifier.issn
0960-3085
dc.identifier.uri
http://hdl.handle.net/11336/98852
dc.description.abstract
Bacterial pectinases degrade the pectic substances present in plant tissues and particularly, polygalacturonases catalyze the hydrolysis of α-(1,4) glycosidic bonds linking D-galacturonic acid units. In this study, polygalacturonase from Streptomyces halstedii ATCC 10897 was immobilized by the matrix entrapment technique using different thermogels. Bacteriological agar added with magnesium cation produced beads with a more stabilized microstructure for enzyme retention, monitored by oscillatory measurements of storage and loss modulus. Agar concentration and protein content were optimized to maximize protein entrapment, product conversion, and reaction yield. Results showed that the mixture at 10:90% (v/v) of protein (2 mg/mL) and agar (4% w/v) was the best immobilization condition to retain 91% of protein and hydrolyze 38% of pectin to allow the highest reaction yield (9.279 g/g) and increase stability up to 48 h of successive reactions. Agarose bead biocatalysts were used in a trickle-bed column operated with recirculation, and this bioreactor allowed the degradation of pear and cucumber residues by enzymatic liquefaction to enhance sugar content up to 15.33 and 9.35 mg/mL, respectively, and decrease viscosity by 92.3%. The scale-up of this process adds value to vegetable residues such as fructooligosaccharides or fermentable sugars, which become a sustainable source of fuels and chemicals.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Institution of Chemical Engineers
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
ENZYME IMMOBILIZATION
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PACKED BED BIOREACTOR
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PEAR AND CUCUMBER RESIDUES
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SACCHARIFICATION
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SCALE-UP
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STREPTOMYCES HALSTEDII ATCC 10897
dc.subject.classification
Otras Biotecnología del Medio Ambiente
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Biotecnología del Medio Ambiente
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Biodegradation of vegetable residues by polygalacturonase-agar using a trickle-bed bioreactor
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-02-18T16:02:39Z
dc.journal.volume
111
dc.journal.pagination
54-61
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Ramírez Tapias, Yuly Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Rivero, Cintia Wanda. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Giraldo Estrada, Catalina. Universidad Eafit; Colombia
dc.description.fil
Fil: Britos, Claudia Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
dc.description.fil
Fil: Trelles, Jorge Abel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Food and Bioproducts Processing
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.fbp.2018.06.006
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960308518304243
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