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dc.contributor.author
Rojas, Natalia Lorena

dc.contributor.author
Lewkowicz, Elizabeth Sandra

dc.contributor.author
Nobile, Matias L.
dc.date.available
2020-03-04T19:20:55Z
dc.date.issued
2018-11
dc.identifier.citation
Rojas, Natalia Lorena; Lewkowicz, Elizabeth Sandra; Nobile, Matias L.; Alternative low-cost process for large-scale production of Bacillus thuringiensis in a simple and novel culture system; Taylor & Francis; Journal of Environmental Science and Health. Part B: Pesticides, Food Contaminants, and Agricultural Wastes; 53; 11; 11-2018; 719-728
dc.identifier.issn
0360-1234
dc.identifier.uri
http://hdl.handle.net/11336/98785
dc.description.abstract
An industrial-scale, profitable method for production of the most widely used bioinsecticide, Bacillus thuringiensis (Bt), is challenging because of its widespread application. The aim of this study is to present a strategy to develop a low-cost, large-scale bioprocess to produce Bt H14. This study was first focused on the design of a culture medium composed of economical and available components, such as glycerol and lysed Saccharomyces cerevisiae. The production goal of 1200 ITU was achieved using a medium composed of 20:20 g L −1 of glycerol:lysed yeast in batch cultures. Efforts were subsequently focused on the design of an appropriate culture system, and an original two-stage culture system was proposed. First, yeast (the primary component of the culture medium) are cultivated using a minimal mineral medium and lysed, and in the second stage, Bt is cultivated in the same bioreactor using the lysed yeasts as culture medium (supplemented with a feeding pulse of 10 g L −1 glycerol). This system was called fed batch one pot (FOP). A new inoculation strategy is also presented in this study, since these Bt cultures were inoculated directly with heat pre-treated spores instead of vegetative bacteria to facilitate the bioprocess. This study was developed from the laboratory to production-scale bioreactors (measuring from 500 mL to 2500 L), and the efficiency of the proposed strategy was evident in LD 50 tests results, achieving 1796 ITU in large-scale processes. Both the use of non-conventional sources and the process development for biomass production are important for cost-effective production of Bt-based insecticides in mosquito control projects.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis

dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BACILLUS THURINGIENSIS ISRAELENSIS
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BIOPESTICIDE
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FED BATCH ONE POT
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GLYCEROL
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SACCHAROMYCES CEREVISIAE
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación

dc.subject.classification
Biotecnología Industrial

dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS

dc.title
Alternative low-cost process for large-scale production of Bacillus thuringiensis in a simple and novel culture system
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-03-04T15:42:32Z
dc.journal.volume
53
dc.journal.number
11
dc.journal.pagination
719-728
dc.journal.pais
Estados Unidos

dc.journal.ciudad
Londres
dc.description.fil
Fil: Rojas, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
dc.description.fil
Fil: Lewkowicz, Elizabeth Sandra. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Química. Laboratorio de Biotransformaciones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Nobile, Matias L.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Área Química. Laboratorio de Biotransformaciones; Argentina
dc.journal.title
Journal of Environmental Science and Health. Part B: Pesticides, Food Contaminants, and Agricultural Wastes

dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1080/03601234.2018.1480156
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/03601234.2018.1480156
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/29869930-alternative-low-cost-process-for-large-scale-production-of-bacillus-thuringiensis-in-a-simple-and-novel-culture-system/
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