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dc.contributor.author
Vásquez López, Alfonso
dc.contributor.author
Palacios Torres, Rogelio Enrique
dc.contributor.author
Montiel-Frausto, Laura Belem
dc.contributor.author
Medero Vega, Víctor Reinaldo
dc.contributor.author
Bernardi Lima, Nelson
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dc.contributor.author
Camacho-Tapia, Moises
dc.contributor.author
Tovar Pedraza, Juan Manuel
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dc.date.available
2020-03-04T13:39:30Z
dc.date.issued
2018-12
dc.identifier.citation
Vásquez López, Alfonso; Palacios Torres, Rogelio Enrique; Montiel-Frausto, Laura Belem; Medero Vega, Víctor Reinaldo; Bernardi Lima, Nelson; et al.; First report of Cladosporium colocasiae causing leaf spot on taro (Colocasia esculenta) in Mexico; American Phytopathological Society; Plant Disease; 102; 12; 12-2018; 2649-2649
dc.identifier.issn
0191-2917
dc.identifier.uri
http://hdl.handle.net/11336/98733
dc.description.abstract
In September 2017, symptoms of leaf spot were observed on approx. 80% of taro (Colocasia esculenta var. antiquorum) plants at a commercial field located in San Juan Bautista Tuxtepec, Oaxaca, Mexico. Lesions on diseased leaves were dark brown and oval to irregular, sometimes coalesced to cover large areas of the leaf surface. To isolate the fungus, small fragments from adjacent tissue to lesions of symptomatic leaves were excised and surface disinfested by immersion in a 1% NaOCl for 2 min, rinsed three times in sterile distilled water, dried in blotter paper, and placed in Petri plates containing potato dextrose agar (PDA) amended with ampicillin (20 mg/l) and rifampicin (20 mg/l). The plates were incubated at 25ºC under constant white light for 8 days and then mycelial plugs were aseptically transferred to fresh PDA medium. Pure cultures were obtained by monoconidial isolation technique. Colonies on PDA exhibited aerial mycelium sparse, grey-olivaceous to darkolivaceous, and with sporulation profuse. Conidiophores were solitary, erect, straight or slightly flexuous, cylindrical-oblong, olivaceous-brown, nodulose, usually unbranched, and measuring up to 650 μm long. Conidia catenate in branched chains, ellipsoidal to cylindrical, smooth, aseptate or 1?2-septate, olivaceous-brown, 9 to 18 × 5 to 8 μm. Based on morphological characteristics, the fungus was identified as Cladosporium colocasiae (Bensch et al. 2012; García and Moya 2015). A representative isolate was deposited in the Culture Collection of Phytopathogenic Fungi at the Chapingo Autonomous University as UACH 291. For molecular identification, the internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α (EF1-α) gene were amplified by PCR, and sequenced using the primer sets ITS5/ITS4 (White et al. 1990) and EF1-728F/EF1-986R (Carbone and Kohn 1999), respectively. The sequences were deposited in GenBank (Accession numbers ITS:MH191366 and EF1-α:MH191367). A phylogenetic analysis using Bayesian inference and including published ITS and EF1-α sequence dataset for C. colocasiae and other Cladosporium species was performed. The phylogenetic analysis resulted in a well-supported clade grouped with the type species of C. colocasiae. The pathogenicity of the fungus was verified on taro plants growing in a greenhouse. Two leaves from each of five 5-month-old taro plants were sprayed with a conidial suspension (104 spores/ml). Five leaves were mock inoculated with distilled water as a control. All plants were covered for 48 h with a plastic bag to keep moisture and then were maintained in a greenhouse at temperatures ranging from 25 to 32°C for 15 days. The pathogenicity test was performed twice. Symptoms of leaf spots were observed after 10 days on all leaves inoculated with conidial suspensions, while control leaves remained symptomless. Koch´s postulates were fulfilled when the pathogen was reisolated 100% from the diseased leaves. Cladosporium colocasiae has been reported on Colocasia spp. in North America (USA), Caribbean islands, South America, Europe, as well as widely distributed in Africa, Asia, and Australasia (Bensch et al. 2012; Farr and Rossman 2018). To our knowledge, this is the first report of C. colocasiae causing leaf spot on taro in Mexico. The occurrence of this pathogen presents a threat to the production of taro in this area of southeastern Mexico, and therefore, effective management strategies should be implemented.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Phytopathological Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
TARO
dc.subject
LEAF SPOT
dc.subject
CLADOSPORIUM COLOCASIAE
dc.subject.classification
Agronomía, reproducción y protección de plantas
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dc.subject.classification
Agricultura, Silvicultura y Pesca
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
First report of Cladosporium colocasiae causing leaf spot on taro (Colocasia esculenta) in Mexico
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-02-19T18:06:42Z
dc.journal.volume
102
dc.journal.number
12
dc.journal.pagination
2649-2649
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
St. Paul
dc.description.fil
Fil: Vásquez López, Alfonso. Instituto Politécnico Nacional; México
dc.description.fil
Fil: Palacios Torres, Rogelio Enrique. Universidad del Papaloapa; México
dc.description.fil
Fil: Montiel-Frausto, Laura Belem. Instituto Politécnico Nacional; México
dc.description.fil
Fil: Medero Vega, Víctor Reinaldo. Instituto de Investigaciones de Viandas Tropicales; Cuba
dc.description.fil
Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.description.fil
Fil: Camacho-Tapia, Moises. Universidad Autonoma Chapingo; México
dc.description.fil
Fil: Tovar Pedraza, Juan Manuel. Centro de Investigación En Alimentación y Desarrollo; México
dc.journal.title
Plant Disease
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-18-0606-PDN
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1094/PDIS-04-18-0606-PDN
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