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dc.contributor.author
Alvarez, Gabriel Martín
dc.contributor.author
Dalvit, Gabriel Carlos
dc.contributor.author
Achi, Maria Veronica
dc.contributor.author
Miguez, Marcelo Sergio
dc.contributor.author
Cetica, Pablo Daniel
dc.date.available
2020-02-26T19:44:08Z
dc.date.issued
2009-12
dc.identifier.citation
Alvarez, Gabriel Martín; Dalvit, Gabriel Carlos; Achi, Maria Veronica; Miguez, Marcelo Sergio; Cetica, Pablo Daniel; Immature oocyte quality and maturational competence of porcine cumulus-oocyte complex subpopulations; Instituto de Histología y Embriología "Dr. Mario Burgos"; Biocell; 33; 3; 12-2009; 167-177
dc.identifier.issn
0327-9545
dc.identifier.uri
http://hdl.handle.net/11336/98478
dc.description.abstract
Porcine immature oocyte quality (i.e., that of live oocytes at the germinal vesicle stage) was evaluated according to features of the surrounding cumulus, aiming to establish maturational competence of different subpopulations of such cumulus-oocyte complexes. Six subpopulations were identified: A1 (with a dense cumulus), A2 (with a translucent cumulus), B1 (with the corona radiata), B2 (partly naked oocytes), C (naked oocytes), D (with a dark cumulus). The percent incidence of live oocyte in these subpopulations changed significantly as related to cumulus features, however the occurrence of oocytes in the germinal vesicle stage was lower in class D only. Similar metaphase II rates achieved in A1, A2, B1 and B2 classes after in vitro maturation suggest that the nucleus may in fact mature in vitro, in spite of the different accompanying cumulus features which are typical of these classes. In contrast, a higher cytoplasmic maturation rate obtained in class A1 may indicate a stronger dependence of this variable upon cumulus features than that shown by nuclear maturation. When different types of cumulus expansion after in vitro maturation were considered (i.e., fully expanded cumulus, partly expanded cumulus, and partly naked oocyte), no differences were found in the percent of oocytes reaching metaphase II or cytoplasmic maturation. It is concluded that morphological features of the collected porcine cumulus-oocyte complexes (rather than cumulus behavior during culture) may be useful for selection of potentially competent oocytes for in vitro fertilization and embryo production.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Instituto de Histología y Embriología "Dr. Mario Burgos"
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
In vitro maturation
dc.subject
Nuclear maturation
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Cytoplasmic maturation
dc.subject
Pig
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Immature oocyte quality and maturational competence of porcine cumulus-oocyte complex subpopulations
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-02-07T13:45:28Z
dc.journal.volume
33
dc.journal.number
3
dc.journal.pagination
167-177
dc.journal.pais
Argentina
dc.description.fil
Fil: Alvarez, Gabriel Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Dalvit, Gabriel Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Achi, Maria Veronica. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Miguez, Marcelo Sergio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.journal.title
Biocell
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/20067032
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