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dc.contributor.author
Lavaisse, Lucía María
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dc.contributor.author
Hollmann, Axel
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dc.contributor.author
Nazareno, Mónica Azucena
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dc.contributor.author
Disalvo, Edgardo Anibal
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dc.date.available
2020-02-21T14:47:07Z
dc.date.issued
2019-02
dc.identifier.citation
Lavaisse, Lucía María; Hollmann, Axel; Nazareno, Mónica Azucena; Disalvo, Edgardo Anibal; Zeta potential changes of Saccharomyces cerevisiae during fermentative and respiratory cycles; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 174; 2-2019; 63-69
dc.identifier.issn
0927-7765
dc.identifier.uri
http://hdl.handle.net/11336/98258
dc.description.abstract
Saccharomyces cerevisiae is a type of yeast, widely used in diverse biotechnological food-beverage processes. Although the performance of an industrial fermentation process depends largely on the number of cells, it is necessary to consider the physiological state of the cultures. In this context, the aim of this study was to determine in a yeast culture how factors such as growth conditions affect surface properties at the different growth stages. Our results show that, S. cerevisiae spp. exhibits different zeta potential mean values along the exponential, post-diauxic and stationary growth phases. In addition, there were differences depending on whether they are in aerobic or anaerobic conditions. When the effect of pH on the media was studied, a different dependence of zeta potential at each stage reveals that in the living cells the surface potential depends on the interaction between secreted acids and the constituents of the surfaces, according to the growth conditions. In order to have a view at the cellular level, the zeta potential on individual cells by optical microscopy has been determined at different stages of culture in aerobic and anaerobic conditions. This single-cell method allows for the identification and following of the development of different cell subpopulations during each growth stage. Furthermore, the behavior of the dead cells provided evidence to relate the large negatively charged population with cell wall damage. Overall, the results obtained in the present work represent an important milestone for a novel application of zeta potential technique on yeast.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AEROBIC AND ANAEROBIC GROWTH
dc.subject
ELECTROPHORETIC MOBILITY
dc.subject
GROWTH PHASE
dc.subject
PH
dc.subject
SACCHAROMYCES CEREVISIAE
dc.subject
ZETA POTENTIAL
dc.subject.classification
Biofísica
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Zeta potential changes of Saccharomyces cerevisiae during fermentative and respiratory cycles
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-02-18T16:10:41Z
dc.journal.volume
174
dc.journal.pagination
63-69
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Lavaisse, Lucía María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero; Argentina
dc.description.fil
Fil: Hollmann, Axel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero; Argentina
dc.description.fil
Fil: Nazareno, Mónica Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero; Argentina
dc.description.fil
Fil: Disalvo, Edgardo Anibal. Universidad Nacional de Santiago del Estero; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Colloids and Surfaces B: Biointerfaces
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S092777651830777X
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.colsurfb.2018.11.001
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