Artículo
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
Canizo, Jesica Romina; Vazquez Echegaray, Camila
; Klisch, Doris; Aller Atucha, Juan Florencio; Paz, Dante Agustin
; Alberio, Ricardo; Alberio, Ramiro; Guberman, Alejandra Sonia
Fecha de publicación:
07/2018
Editorial:
BioMed Central Ltd.
Revista:
BMC Research Notes
ISSN:
1756-0500
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. Results: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells.
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Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Canizo, Jesica Romina; Vazquez Echegaray, Camila; Klisch, Doris; Aller Atucha, Juan Florencio; Paz, Dante Agustin; et al.; Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system; BioMed Central Ltd.; BMC Research Notes; 11; 1; 7-2018; 1-8
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