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dc.contributor.author
Susuki, Keiichiro
dc.contributor.author
Zollinger, Daniel R.
dc.contributor.author
Chang, Kae Jiun
dc.contributor.author
Zhang, Chuansheng
dc.contributor.author
Huang, Claire Yu Mei
dc.contributor.author
Tsai, Chang Ru
dc.contributor.author
Galiano, Mauricio Raul
dc.contributor.author
Liu, Yanhong
dc.contributor.author
Benusa, Savannah D.
dc.contributor.author
Yermakov, Leonid M.
dc.contributor.author
Griggs, Ryan B.
dc.contributor.author
Dupree, Jeffrey L.
dc.contributor.author
Rasband, Matthew N.
dc.date.available
2020-02-18T17:59:19Z
dc.date.issued
2018-07
dc.identifier.citation
Susuki, Keiichiro; Zollinger, Daniel R.; Chang, Kae Jiun; Zhang, Chuansheng; Huang, Claire Yu Mei; et al.; Glial βii spectrin contributes to paranode formation and maintenance; Society for Neuroscience; Journal of Neuroscience; 38; 27; 7-2018; 6063-6075
dc.identifier.issn
0270-6474
dc.identifier.uri
http://hdl.handle.net/11336/97921
dc.description.abstract
Action potential conduction along myelinated axons depends on high densities of voltage-gated Na channels at the nodes of Ranvier. Flanking each node, paranodal junctions (paranodes) are formed between axons and Schwann cells in the peripheral nervous system (PNS) or oligodendrocytes intheCNS. Paranodal junctions contribute to both no deassembly and maintenance. Despitetheir importance, the molecular mechanisms responsible for paranode assembly and maintenance remain poorly understood. βII spectrin is expressed in diverse cells and is an essential part of the submembranous cytoskeleton. Here, we show that Schwann cell βII spectrin is highly enriched at paranodes. To elucidate the roles of glial βII spectrin, we generated mutant mice lacking βII spectrin in myelinating glial cells by crossing mice with a floxed allele of Sptbn1 with Cnp-Cre mice, and analyzed both male and female mice. Juvenile (4 weeks) and middle-aged (60 weeks) mutant mice showed reduced grip strength and sciatic nerve conduction slowing, whereas no phenotype was observed between 8 and 24 weeks of age. Consistent with these findings, immunofluorescence microscopy revealed disorganized paranodes in the PNS and CNS of both postnatal day 13 and middle-aged mutant mice, but not in young adult mutant mice. Electron microscopy confirmed partial loss of transverse bands at the paranodal axoglial junction in the middle-aged mutant mice in both the PNS and CNS. These findings demonstrate that a spectrin-based cytoskeleton in myelinating glia contributes to formation and maintenance of paranodal junctions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society for Neuroscience
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
MYELIN
dc.subject
NODE OF RANVIER
dc.subject
PARANODE
dc.subject
SPECTRIN
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Glial βii spectrin contributes to paranode formation and maintenance
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-22T16:44:19Z
dc.journal.volume
38
dc.journal.number
27
dc.journal.pagination
6063-6075
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Susuki, Keiichiro. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: Zollinger, Daniel R.. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: Chang, Kae Jiun. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: Zhang, Chuansheng. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: Huang, Claire Yu Mei. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: Tsai, Chang Ru. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: Galiano, Mauricio Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: Liu, Yanhong. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: Benusa, Savannah D.. Virginia Commonwealth University; Estados Unidos
dc.description.fil
Fil: Yermakov, Leonid M.. Wright State University; Estados Unidos
dc.description.fil
Fil: Griggs, Ryan B.. Wright State University; Estados Unidos
dc.description.fil
Fil: Dupree, Jeffrey L.. Virginia Commonwealth University; Estados Unidos
dc.description.fil
Fil: Rasband, Matthew N.. Baylor College of Medicine; Estados Unidos
dc.journal.title
Journal of Neuroscience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.3647-17.2018
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1523/JNEUROSCI.3647-17.2018
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