Artículo
Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat
Zurvarra, Francisco Miguel; Salvetti, Natalia Raquel
; Mason, J. Ian; Velázquez, Melisa María del Luján
; Alfaro, Natalia Soledad
; Ortega, Hugo Hector
Fecha de publicación:
12/2009
Editorial:
Csiro Publishing
Revista:
Reproduction Fertility and Development
ISSN:
1031-3613
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The objective of the present study was to characterise the expression and tissue distribution of steroid receptors (oestrogen receptor- and (ER, ER), androgen receptor (AR) and progesterone receptor (PR)) and steroidogenic enzymes (P450 aromatase (P450arom), 3-hydroxysteroid dehydrogenase (3-HSD) and steroidogenic acute regulatory protein (StAR)) in letrozole-induced polycystic ovaries of rats. Changes in serum hormone levels, protein expression in whole ovaries by western blot analysis and protein localisation by immunohistochemistry were determined in female rats treated with the aromatase inhibitor letrozole and compared with controls in proestrous and diestrous rats. Increases in the serum LH, FSH and testosterone concentrations were observed in letrozole-treated rats whereas serum oestradiol and progesterone levels were reduced. Protein expression as analysed by western immunoblot was consistent with the immunohistochemical data. Letrozole treatment induced an increase in the expression of AR, StAR and 3-HSD and a decrease in ER. ER, PR and P450arom showed partial changes in relation to some cycle stages. These results indicate that cystogenesis in this experimental model is characterised by changes in steroid receptors and steroidogenic enzyme expression that may be essential to proper ovarian functioning and are in agreement with similar changes observed in women with PCOS.
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Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Zurvarra, Francisco Miguel; Salvetti, Natalia Raquel; Mason, J. Ian; Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; et al.; Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozole-induced polycystic ovaries in rat; Csiro Publishing; Reproduction Fertility and Development; 21; 7; 12-2009; 827-839
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