Artículo

Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode

Saidman, Silvana BeatrizIcon ; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.
Fecha de publicación: 11/2000
Editorial: Elsevier Science
Revista: Analytica Chimica Acta
ISSN: 0003-2670
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

A biosensor based on modified carbon paste electrodes for the amperometric detection of D-sorbitol is described. Carbon paste electrodes were modified by D-sorbitol dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NAD+) and coated with a non-conducting poly(o-phenylenediamine) (PPD) film. After partial oxidation of the immobilised NAD+ by applying a potential step f rom 0.0 to 1.2 V (SCE), the modified electrode allowed the amperometric detection of the NADH enzymatically obtained at an applied potential of 0.0 V. The resulting biosensor responded rapidly to sorbitol up to 8 x 10-4 M with a detection limit of 4 x 10-5 M.
Palabras clave: AMPEROMETRY , NAD+ OXIDATION , SORBITOL BIOSENSOR
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/97809
DOI: https://doi.org/10.1016/S0003-2670(00)01140-5
URL: https://www.sciencedirect.com/science/article/pii/S0003267000011405
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Articulos(CCT - BAHIA BLANCA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - BAHIA BLANCA
Citación
Saidman, Silvana Beatriz; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.; Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode; Elsevier Science; Analytica Chimica Acta; 424; 1; 11-2000; 45-50
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