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dc.contributor.author
Velázquez, Melisa María del Luján
dc.contributor.author
Alfaro, Natalia Soledad
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Dupuy, Carlos
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Salvetti, Natalia Raquel
dc.contributor.author
Rey, Florencia
dc.contributor.author
Ortega, Hugo Hector
dc.date.available
2020-02-14T20:45:36Z
dc.date.issued
2010-04
dc.identifier.citation
Velázquez, Melisa María del Luján; Alfaro, Natalia Soledad; Dupuy, Carlos; Salvetti, Natalia Raquel; Rey, Florencia; et al.; Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease; Elsevier Science; Animal Reproduction Science; 118; 2-4; 4-2010; 201-209
dc.identifier.issn
0378-4320
dc.identifier.uri
http://hdl.handle.net/11336/97692
dc.description.abstract
The present study was performed to determine how the development of cystic ovarian disease (COD) affecting the ovarian expression of heat shock proteins (HSP) in cows were expressing extrous cycles. HSP27, HSP60, HSP70 and HSP90 were evaluated in different ovarian components by Western blot and semiquantitative immunohistochemical analysis. Greater expression of the HSP27 gene was detected in the granulosa and theca cells of primary, secondary, tertiary and cystic follicles, with decreasing amount in atretic follicles. HSP60, HSP70 and HSP90 showed a similar pattern of immunostaining, with moderate gene expression in primary and secondary follicles, increased expression in tertiary and atretic follicles with the greatest gene expression in cystic follicles. HSP were also localized in the corpus luteum, corpus albicans, interstitial tissue and tunica albuginea. The relative amount of protein in the follicular wall of small and large healthy follicles and cystic follicles as analysed by Western immunoblot was consistent with the immunohistochemical data. We speculate that altered expression of HSP genes decreases apoptosis in the follicular wall and leads to the delayed regression of cystic follicles. This study supports earlier observations suggesting that aberrant HSP gene expression, observed in cells of the cystic follicles, is probably associated with the intra-ovarian component of COD pathogenesis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CYSTIC OVARIAN DISEASE
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HEAT SHOCK PROTEINS (HSP)
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OVARY
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Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-02-13T18:58:27Z
dc.journal.volume
118
dc.journal.number
2-4
dc.journal.pagination
201-209
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Velázquez, Melisa María del Luján. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Alfaro, Natalia Soledad. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Dupuy, Carlos. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Rey, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Animal Reproduction Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.anireprosci.2009.08.010
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