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Artículo

Rapid methodology for antigenic profiling of FMDV field strains and for the control of identity, purity and viral integrity in commercial virus vaccines using monoclonal antibodies

Seki, CristinaIcon ; Robiolo, Blanca; Periolo, Osvaldo HoracioIcon ; Iglesias, Nelida MarcelaIcon ; D'antuono, Alejandra LorenaIcon ; Maradei, Eduardo; Barros, Virginia; la Torre, Jose LeonardoIcon ; Mattion, Nora MartaIcon
Fecha de publicación: 01/2009
Editorial: Elsevier Science
Revista: Veterinary Microbiology
ISSN: 0378-1135
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Virología; Ciencias Veterinarias

Resumen

Monoclonal antibodies (MAbs) developed against different foot-and-mouth disease virus (FMDV) vaccine strains were extensively used to study any possible antigenic variations during vaccine production in Argentine facilities. Additionally, a typing ELISA using strain specific MAbs was developed to detect potential cross contaminations among FMDV strains in master and working seeds with high specificity and sensitivity and to confirm strains identity in formulated vaccines. This assay was carried out for the South American strains currently in use in production facilities in Argentina (A24/Cruzeiro, A/Argentina/01, O1/Campos and C3/Indaial) and for the strain O/Taiwan, produced only for export to Asia. These non-cross reactive MAbs were also used to analyze the integrity of viral particles belonging to each one of the individual strains, following isolation of 140S virions by means of sucrose density gradients from the aqueous phase of commercial polyvalent vaccines. Antigenic profiles were defined for FMDV reference strains using panels of MAbs, and a coefficient of correlation of reactivity with these panels was calculated to establish consistent identity upon serial passages of master and production seeds. A comparison of vaccine and field strain antigenic profiles performed using coefficients of correlation allowed the rapid identification of two main groups of serotype A viruses collected during the last FMD epidemic in Argentina, whose reactivity matched closely to A/Argentina/2000 and A/Argentina/2001 strains.
Palabras clave: ANTIGENIC PROFILES , FMDV , MONOCLONAL ANTIBODIES , TRAPPING ELISA , VACCINE CONTROL , VIRAL INTEGRITY
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/97474
URL: https://www.sciencedirect.com/science/article/pii/S0378113508002782
DOI: http://dx.doi.org/10.1016/j.vetmic.2008.07.011
Colecciones
Articulos(ICT - MILSTEIN)
Articulos de INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN"
Citación
Seki, Cristina; Robiolo, Blanca; Periolo, Osvaldo Horacio; Iglesias, Nelida Marcela; D'antuono, Alejandra Lorena; et al.; Rapid methodology for antigenic profiling of FMDV field strains and for the control of identity, purity and viral integrity in commercial virus vaccines using monoclonal antibodies; Elsevier Science; Veterinary Microbiology; 133; 3; 1-2009; 239-251
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