Artículo
The tolC locus affects the expression of sbmA through σE activity increase.
Corbalan, Natalia Soledad
; Adler, Conrado
; de Cristobal, Ricardo Ezequiel
; Pomares, Maria Fernanda
; Delgado, Monica Alejandra
; Vincent, Paula Andrea
Fecha de publicación:
10/2010
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
FEMS Microbiology Letters
ISSN:
0378-1097
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The SbmA protein is involved in the transport of MccB17-, MccJ25-, bleomycin- and proline-rich peptides into the Escherichia coli cytoplasm. sbmA gene homologues were found in a variety of bacteria. However, the physiological role of this protein still remains unknown. Previously, we found that a combination of sbmA and tolC mutations in Tn10-carrying E. coli K-12 strains results in hypersusceptibility to tetracycline. In this work, we studied sbmA expression in a tolC mutant background and observed an increased expression throughout growth. We ruled out the global transcriptional regulator RpoS and the small RNA micF as intermediates in this regulation. The tolC mutation induced the expression of other well-characterized strong σE- dependent promoters in E. coli. We observed that the increase in σE activity led to a greater sbmA expression, conversely eliminating σE prevented expression of sbmA. We also observed that the sbmA upregulation in a tolC mutant context was abolished in an rpoE-null strain. These results suggest a σE-dependent positive regulation on sbmA by the tolC mutation. We hypothesize that this mechanism might be part of a compensatory cell envelope stress response. © 2010 Federation of European Microbiological Societies.
Palabras clave:
ΣE
,
SBMA REGULATION
,
TOLC
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Identificadores
Colecciones
Articulos(CCT - NOA SUR)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Corbalan, Natalia Soledad; Adler, Conrado; de Cristobal, Ricardo Ezequiel; Pomares, Maria Fernanda; Delgado, Monica Alejandra; et al.; The tolC locus affects the expression of sbmA through σE activity increase.; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 311; 2; 10-2010; 185-192
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