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dc.contributor.author
Sorroche, Fernando Guido
dc.contributor.author
Spesia, Mariana Belen
dc.contributor.author
Zorreguieta, Ángeles
dc.contributor.author
Giordano, Walter Fabian
dc.date.available
2020-02-10T17:21:57Z
dc.date.issued
2012-06
dc.identifier.citation
Sorroche, Fernando Guido; Spesia, Mariana Belen; Zorreguieta, Ángeles; Giordano, Walter Fabian; A positive correlation between bacterial autoaggregation and biofilm formation in Native Sinorhizobium meliloti isolates from Argentina; American Society for Microbiology; Applied And Environmental Microbiology; 78; 12; 6-2012; 4092-4101
dc.identifier.issn
0099-2240
dc.identifier.uri
http://hdl.handle.net/11336/97067
dc.description.abstract
Sinorhizobium meliloti is a symbiotic nitrogen-fixing bacterium that elicits nodule formation on roots of alfalfa plants. S. meliloti produces two exopolysaccharides (EPSs), termed EPS I and EPS II, that are both able to promote symbiosis. EPS I and EPS II are secreted in two major fractions that reflect differing degrees of subunit polymerization, designated high- and low-molecular-weight fractions. We reported previously that EPSs are crucial for autoaggregation and biofilm formation in S. meliloti reference strains and isogenic mutants. However, the previous observations were obtained by use of ?domesticated? laboratory strains, with mutations resulting from successive passages under unnatural conditions, as has been documented for reference strain Rm1021. In the present study, we analyzed the autoaggregation and biofilm formation abilities of native S. meliloti strains isolated from root nodules of alfalfa plants grown in four regions of Argentina. 16S rRNA gene analysis of all the native isolates revealed a high degree of identity with reference S. meliloti strains. PCR analysis of the expR gene of all the isolates showed that, as in the case of reference strain Rm8530, this gene is not interrupted by an insertion sequence (IS) element. A positive correlation was found between autoaggregation and biofilm formation abilities in these rhizobia, indicating that both processes depend on the same physical adhesive forces. Extracellular complementation experiments using mutants of the native strains showed that autoaggregation was dependent on EPS II production. Our results indicate that a functional EPS II synthetic pathway and its proper regulation are essential for cell-cell interactions and surface attachment of S. meliloti.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
RHIZOBIUM
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BIOFILM
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AUTOAGGREGATION
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EXOPOLYSACCHARIDE
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A positive correlation between bacterial autoaggregation and biofilm formation in Native Sinorhizobium meliloti isolates from Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-02-07T13:37:35Z
dc.identifier.eissn
1098-5336
dc.journal.volume
78
dc.journal.number
12
dc.journal.pagination
4092-4101
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Sorroche, Fernando Guido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina
dc.description.fil
Fil: Spesia, Mariana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina
dc.description.fil
Fil: Zorreguieta, Ángeles. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Instituto de Invest.bioquímicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina
dc.journal.title
Applied And Environmental Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/78/12/4092.long
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1128%2FAEM.07826-11
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