Artículo
Flow injection analysis: Rayleigh light scattering technique for total protein determination
Vidal, Ezequiel Martin; Palomeque, Miriam Edid; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana
Fecha de publicación:
12/2003
Editorial:
Springer Heidelberg
Revista:
Analytical and Bioanalytical Chemistry
ISSN:
1618-2642
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A novel flow injection analysis (FIA) method with Rayleigh light scattering (RLS) detection was developed for the determination of total protein concentrations. This method is based on the weak intensity of RLS of bromothymol blue (BB) (3′,3″-dibromothymolsulfonephthalein) which can be enhanced by the addition of protein in weakly acidic solution. A common spectrofluorimeter was used as a detector. It was proved that the application of this method to quantify the total proteins in real samples by using bovine serum albumin was possible. The RLS signal was detected at λ ex=λ em=572 nm. The linear range was 7.0-70.0 μg mL-1, the detection limit was 3.75 μg mL-1, the reproducibility was 5.5% (n=7), and the sample throughput was 26 h-1.
Palabras clave:
FIA
,
PROTEINS
,
RAYLEIGH LIGHT SCATTERING
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INQUISUR)
Articulos de INST.DE QUIMICA DEL SUR
Articulos de INST.DE QUIMICA DEL SUR
Citación
Vidal, Ezequiel Martin; Palomeque, Miriam Edid; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Flow injection analysis: Rayleigh light scattering technique for total protein determination; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 376; 1; 12-2003; 38-41
Compartir
Altmétricas