Mostrar el registro sencillo del ítem
dc.contributor.author
Zschach, Henrike
dc.contributor.author
Larsen, Mette V.
dc.contributor.author
Hasman, Henrik
dc.contributor.author
Westh, Henrik
dc.contributor.author
Nielsen, Morten
dc.contributor.author
Miedzybrodzki, Ryszard
dc.contributor.author
Jónczyk Matysiak, Ewa
dc.contributor.author
Weber-Dabrowska, Beata
dc.contributor.author
Górski, Andrzej
dc.date.available
2020-02-07T19:00:40Z
dc.date.issued
2018-03
dc.identifier.citation
Zschach, Henrike; Larsen, Mette V.; Hasman, Henrik; Westh, Henrik; Nielsen, Morten; et al.; Use of a regression model to study host-genomic determinants of phage susceptibility in MRSA; MDPI; Antibiotics; 7; 1; 3-2018; 1-16
dc.identifier.issn
2079-6382
dc.identifier.uri
http://hdl.handle.net/11336/96913
dc.description.abstract
Staphylococcus aureus is a major agent of nosocomial infections. Especially in methicillinresistant strains, conventional treatment options are limited and expensive, which has fueled a growing interest in phage therapy approaches. We have tested the susceptibility of 207 clinical S. aureus strains to 12 (nine monovalent) different therapeutic phage preparations and subsequently employed linear regression models to estimate the influence of individual host gene families on resistance to phages. Specifically, we used a two-step regression model setup with a preselection step based on gene family enrichment. We show that our models are robust and capture the data’s underlying signal by comparing their performance to that of models build on randomized data. In doing so, we have identified 167 gene families that govern phage resistance in our strain set and performed functional analysis on them. This revealed genes of possible prophage or mobile genetic element origin, along with genes involved in restriction-modification and transcription regulators, though the majority were genes of unknown function. This study is a step in the direction of understanding the intricate host-phage relationship in this important pathogen with the outlook to targeted phage therapy applications.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BACTERIAL PHAGE RESISTANCE
dc.subject
MRSA
dc.subject
PHAGE THERAPY
dc.subject
REGRESSION MODELING
dc.subject.classification
Otras Ciencias de la Salud
dc.subject.classification
Ciencias de la Salud
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Use of a regression model to study host-genomic determinants of phage susceptibility in MRSA
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-11-25T17:45:31Z
dc.journal.volume
7
dc.journal.number
1
dc.journal.pagination
1-16
dc.journal.pais
Suiza
dc.journal.ciudad
Basilea
dc.description.fil
Fil: Zschach, Henrike. Technical University of Denmark; Dinamarca
dc.description.fil
Fil: Larsen, Mette V.. Goseqit Aps; Dinamarca
dc.description.fil
Fil: Hasman, Henrik. Statens Serum Institut; Dinamarca
dc.description.fil
Fil: Westh, Henrik. Hvidovre Hospital; Dinamarca. Universidad de Copenhagen; Dinamarca
dc.description.fil
Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: Miedzybrodzki, Ryszard. Polish Academy Of Sciences. Ludwik Hirszfeld Institute of Immunology and Experimental Therapy; Polonia. Medical University Of Warsaw; Polonia
dc.description.fil
Fil: Jónczyk Matysiak, Ewa. Polish Academy Of Sciences. Ludwik Hirszfeld Institute of Immunology and Experimental Therapy; Polonia
dc.description.fil
Fil: Weber-Dabrowska, Beata. Polish Academy Of Sciences. Ludwik Hirszfeld Institute of Immunology and Experimental Therapy; Polonia
dc.description.fil
Fil: Górski, Andrzej. Medical University Of Warsaw; Polonia. Polish Academy Of Sciences. Ludwik Hirszfeld Institute of Immunology and Experimental Therapy; Polonia
dc.journal.title
Antibiotics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/antibiotics7010009
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6382/7/1/9
Archivos asociados