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dc.contributor.author
Musri, Melina Mara
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dc.contributor.author
Coll Bonfill, Núria
dc.contributor.author
Maron, Bradley A.
dc.contributor.author
Peinado, Victor Ivo
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dc.contributor.author
Wang, Rui Sheng
dc.contributor.author
Altirriba, Jordi
dc.contributor.author
Blanco, Isabel
dc.contributor.author
Oldham, William M.
dc.contributor.author
Tura Ceide, Olga
dc.contributor.author
García Lucio, Jessica
dc.contributor.author
de la Cruz Thea, Benjamín Isaías
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dc.contributor.author
Meister, Gunter
dc.contributor.author
Loscalzo, Joseph
dc.contributor.author
Barberà, Joan A.
dc.date.available
2020-02-07T18:05:33Z
dc.date.issued
2018-10
dc.identifier.citation
Musri, Melina Mara; Coll Bonfill, Núria; Maron, Bradley A.; Peinado, Victor Ivo; Wang, Rui Sheng; et al.; MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling; American Thoracic Society; American Journal Of Respiratory Cell And Molecular Biology; 59; 4; 10-2018; 490-499
dc.identifier.issn
1044-1549
dc.identifier.uri
http://hdl.handle.net/11336/96868
dc.description.abstract
Pulmonary vascular remodeling is an angiogenic-related process involving changes in smooth muscle cell (SMC) homeostasis, which is frequently observed in chronic obstructive pulmonary disease (COPD). MicroRNAs (miRNAs) are small, noncoding RNAs that regulate mRNA expression levels of many genes, leading to the manifestation of cell identity and specific cellular phenotypes. Here, we evaluate the miRNA expression profiles of pulmonary arteries (PAs) of patients with COPD and its relationship with the regulation of SMC phenotypic change. miRNA expression profiles from PAs of 12 patients with COPD, 9 smokers with normal lung function (SK), and 7 nonsmokers (NS) were analyzed using TaqMan Low-Density Arrays. In patients with COPD, expression levels of miR-98, miR- 139-5p, miR-146b-5p, and miR-451 were upregulated, as compared with NS. In contrast, miR-197, miR-204, miR-485-3p, and miR-627 were downregulated. miRNA-197 expression correlated with both airflowobstruction andPAintimal enlargement. In an in vitro model of SMC differentiation, miR-197 expression was associated with an SMC contractile phenotype. miR-197 inhibition blocked the acquisition of contractile markers in SMCs and promoted a proliferative/migratory phenotypemeasured by both cell cycle analysis and wound-healing assay. Using luciferase assays, Western blot, and quantitative PCR, we confirmed that miR-197 targets the transcription factor E2F1. In PAs from patients with COPD, levels of E2F1 were increased as compared withNS. InPAs of patients with COPD, remodeling of the vesselwall is associated with downregulation of miR-197, which regulates SMC phenotype. The effect ofmiR-197 onPAsmight bemediated, at least in part, by the key proproliferative factor, E2F1.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Thoracic Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
COPD
dc.subject
MICRORNAS
dc.subject
PULMONARY ARTERY
dc.subject
SMOOTH MUSCLE CELL PHENOTYPIC SWITCH
dc.subject
VASCULAR REMODELING
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
MicroRNA dysregulation in pulmonary arteries from chronic obstructive pulmonary disease: Relationships with vascular remodeling
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-23T14:26:22Z
dc.journal.volume
59
dc.journal.number
4
dc.journal.pagination
490-499
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Musri, Melina Mara. Universidad de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil
Fil: Coll Bonfill, Núria. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
dc.description.fil
Fil: Maron, Bradley A.. Brigham And Women's Hospital; Estados Unidos
dc.description.fil
Fil: Peinado, Victor Ivo. Universidad de Barcelona; España. Biomedical Research Centre Network For Respiratory Diseases; España
dc.description.fil
Fil: Wang, Rui Sheng. Brigham And Women's Hospital; Estados Unidos
dc.description.fil
Fil: Altirriba, Jordi. Universidad de Ginebra; Suiza
dc.description.fil
Fil: Blanco, Isabel. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
dc.description.fil
Fil: Oldham, William M.. Brigham And Women's Hospital; Estados Unidos
dc.description.fil
Fil: Tura Ceide, Olga. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
dc.description.fil
Fil: García Lucio, Jessica. Universidad de Barcelona; España
dc.description.fil
Fil: de la Cruz Thea, Benjamín Isaías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
dc.description.fil
Fil: Meister, Gunter. Universitat Regensburg; Alemania
dc.description.fil
Fil: Loscalzo, Joseph. Brigham And Women's Hospital; Estados Unidos
dc.description.fil
Fil: Barberà, Joan A.. Biomedical Research Centre Network For Respiratory Diseases; España. Universidad de Barcelona; España
dc.journal.title
American Journal Of Respiratory Cell And Molecular Biology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1165/rcmb.2017-0040OC
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.atsjournals.org/doi/10.1165/rcmb.2017-0040OC
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