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dc.contributor.author
Corbat, Agustín Andrés
dc.contributor.author
Schuermann, Klaus C.
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Liguzinski, Piotr
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Radon, Yvonne
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Bastiaens, Philippe I.H.
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Verveer, Peter J.
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Grecco, Hernan Edgardo
dc.date.available
2020-02-04T21:33:16Z
dc.date.issued
2018-10
dc.identifier.citation
Corbat, Agustín Andrés; Schuermann, Klaus C.; Liguzinski, Piotr; Radon, Yvonne; Bastiaens, Philippe I.H.; et al.; Co-imaging extrinsic, intrinsic and effector caspase activity by fluorescence anisotropy microscopy; Elsevier; Redox Biology; 19; 10-2018; 210-217
dc.identifier.issn
2213-2317
dc.identifier.uri
http://hdl.handle.net/11336/96720
dc.description.abstract
In order to overcome intercellular variability and thereby effectively assess signal propagation in biological networks it is imperative to simultaneously quantify multiple biological observables in single living cells. While fluorescent biosensors have been the tool of choice to monitor the dynamics of protein interaction and enzymatic activity, co-measuring more than two of them has proven challenging. In this work, we designed three spectrally separated anisotropy-based Förster Resonant Energy Transfer (FRET) biosensors to overcome this difficulty. We demonstrate this principle by monitoring the activation of extrinsic, intrinsic and effector caspases upon apoptotic stimulus. Together with modelling and simulations we show that time of maximum activity for each caspase can be derived from the anisotropy of the corresponding biosensor. Such measurements correlate relative activation times and refine existing models of biological signalling networks, providing valuable insight into signal propagation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
ANISOTROPY FRET BIOSENSOR
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APOPTOTIC NETWORK
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CASPASE ACTIVITY
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CO-MONITORING
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IMAGING
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POLARIZATION MICROSCOPY
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Óptica
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Ciencias Físicas
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CIENCIAS NATURALES Y EXACTAS
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Co-imaging extrinsic, intrinsic and effector caspase activity by fluorescence anisotropy microscopy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-22T18:01:35Z
dc.journal.volume
19
dc.journal.pagination
210-217
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Corbat, Agustín Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
dc.description.fil
Fil: Schuermann, Klaus C.. Institut Max Planck fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Liguzinski, Piotr. Institut Max Planck fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Radon, Yvonne. Institut Max Planck fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Bastiaens, Philippe I.H.. Institut Max Planck fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Verveer, Peter J.. Institut Max Planck fur Molekulare Physiologie; Alemania
dc.description.fil
Fil: Grecco, Hernan Edgardo. Institut Max Planck fur Molekulare Physiologie; Alemania. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
dc.journal.title
Redox Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2213231718305524
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.redox.2018.07.023
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