Artículo
Synaptic control of mRNA translation by reversible assembly of XRN1 bodies
Fecha de publicación:
04/2015
Editorial:
Company of Biologists
Revista:
Journal of Cell Science
ISSN:
0021-9533
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Repression of mRNA translation is linked to the formation of specific cytosolic foci such as stress granules and processing bodies, which store or degrade mRNAs. In neurons, synaptic activity regulates translation at the post-synapse and this is important for plasticity. N-methyl-D-aspartate (NMDA) receptor stimulation downregulates translation, and we speculate that this is linked to the formation of unknown mRNA-silencing foci. Here, we show that the 5'-3' exoribonuclease XRN1 forms discrete clusters associated with the post-synapse that are different from processing bodies or stress granules, and we named them synaptic XRN1 bodies (SX-bodies). Using primary neurons, we found that the SX-bodies respond to synapse stimulation and that their formation correlates inversely with the local translation rate. SX-bodies increase in size and number upon NMDA stimulation, and metabotropic glutamate receptor activation provokes SX-body dissolution, along with increased translation. The response is specific and the previously described Smaug1 foci and FMRP granules show a different response. Finally, XRN1 knockdown impairs the translational repression triggered by NMDA. Collectively, these observations support a role for the SX-bodies in the reversible masking and silencing of mRNAs at the synapse.
Palabras clave:
P BODY
,
RNA GRANULE
,
STRESS GRANULE
,
SYNAPSE
,
XRN1
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Luchelli, Luciana; Thomas, Maria Gabriela; Boccaccio, Graciela Lidia; Synaptic control of mRNA translation by reversible assembly of XRN1 bodies; Company of Biologists; Journal of Cell Science; 128; 8; 4-2015; 1542-1554
Compartir
Altmétricas