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dc.contributor.author
Mohedano, Mari Luz  
dc.contributor.author
Hernández Recio, Sara  
dc.contributor.author
Yépez, Alba  
dc.contributor.author
Requena, Teresa  
dc.contributor.author
Martínez Cuesta, M. Carmen  
dc.contributor.author
Peláez, Carmen  
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Russo, Pasquale  
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Leblanc, Jean Guy Joseph  
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Spano, Giuseppe  
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Aznar, Rosa  
dc.contributor.author
López, Paloma  
dc.date.available
2020-02-03T20:44:29Z  
dc.date.issued
2019-07-31  
dc.identifier.citation
Mohedano, Mari Luz; Hernández Recio, Sara; Yépez, Alba; Requena, Teresa; Martínez Cuesta, M. Carmen; et al.; Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling; Frontiers Media S.A.; Frontiers in Microbiology; 10; 31-7-2019; 1-13  
dc.identifier.issn
1664-302X  
dc.identifier.uri
http://hdl.handle.net/11336/96617  
dc.description.abstract
Some strains of lactic acid bacteria (LAB) produce riboflavin, a water-soluble vitamin of the B complex, essential for human beings. Here, we have evaluated riboflavin (B2 vitamin) production by five Lactobacillus plantarum strains isolated from chicha, a traditional maize-based fermented alcoholic beverage from north-western Argentina and their isogenic riboflavin-overproducing derivatives previously selected using roseoflavin. A direct fluorescence spectroscopic detection method to quantify riboflavin production in bacterial culture supernatants has been tested. Comparison of the efficiency for riboflavin fluorescence quantification with and without prior HPLC fractionation showed that the developed method is a rapid and easy test for selection of B2 vitamin-producing strains. In addition, it can be used for quantitative detection of the vitamin production in real time during bacterial growth. On the basis of this and previous analyses, the L. plantarum M5MA1-B2 riboflavin overproducer was selected for in vitro and in vivo studies after being fluorescently labeled by transfer of the pRCR12 plasmid, which encodes the mCherry protein. The labeling did not affect negatively the growth, the riboflavin production nor the adhesion of the strain to Caco-2 cells. Thus, L. plantarum M5MA1-B2[pRCR12] was evaluated for its survival under digestive tract stresses in the presence of microbiota in the dynamic multistage BFBL gut model and in a murine model. After exposure to both models, M5MA1-B2[pRCR12] could be recovered and detected by the pink color of the colonies. The results indicated a satisfactory resistance of the strain to gastric and intestinal stress conditions but a low colonization capability observed both in vitro and in vivo. Overall, L. plantarum M5MA1-B2 could be proposed as a probiotic strain for the development of functional foods.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Frontiers Media S.A.  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
FLUORESCENT LABELING  
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LACTIC ACID BACTERIA  
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LACTOBACILLUS PLANTARUM  
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PROBIOTIC  
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RIBOFLAVIN  
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Alimentos y Bebidas  
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Otras Ingenierías y Tecnologías  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Real-time detection of riboflavin production by Lactobacillus plantarum strains and tracking of their gastrointestinal survival and functionality in vitro and in vivo using mCherry labeling  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-12-11T20:18:43Z  
dc.journal.volume
10  
dc.journal.pagination
1-13  
dc.journal.pais
Suiza  
dc.journal.ciudad
Lausana  
dc.description.fil
Fil: Mohedano, Mari Luz. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España  
dc.description.fil
Fil: Hernández Recio, Sara. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España  
dc.description.fil
Fil: Yépez, Alba. Universidad de Valencia; España  
dc.description.fil
Fil: Requena, Teresa. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España  
dc.description.fil
Fil: Martínez Cuesta, M. Carmen. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España  
dc.description.fil
Fil: Peláez, Carmen. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España  
dc.description.fil
Fil: Russo, Pasquale. Università di Foggia; Italia  
dc.description.fil
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina  
dc.description.fil
Fil: Spano, Giuseppe. Università di Foggia; Italia  
dc.description.fil
Fil: Aznar, Rosa. Universidad de Valencia; España. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España  
dc.description.fil
Fil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España  
dc.journal.title
Frontiers in Microbiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.01748/full  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2019.01748