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dc.contributor.author
Gómez Gómez, Beatriz
dc.contributor.author
Pérez Corona, Teresa
dc.contributor.author
Mozzi, Fernanda Beatriz
dc.contributor.author
Pescuma, Micaela
dc.contributor.author
Madrid Albarrn, Yolanda
dc.date.available
2020-02-03T20:29:07Z
dc.date.issued
2019-03
dc.identifier.citation
Gómez Gómez, Beatriz; Pérez Corona, Teresa; Mozzi, Fernanda Beatriz; Pescuma, Micaela; Madrid Albarrn, Yolanda; Silac-based quantitative proteomic analysis of Lactobacillus reuteri CRL 1101 response to the presence of selenite and selenium nanoparticles; Elsevier Science; Journal Of Proteomics; 195; 3-2019; 53-65
dc.identifier.issn
1874-3919
dc.identifier.uri
http://hdl.handle.net/11336/96604
dc.description.abstract
Stable isotope labeling in cell culture (SILAC) was applied for the first time on a lactic acid bacterium strain (L. reuteri CRL1101) for analyzing differential protein expression associated to selenite(Na 2 SeO 3 ) and selenium nanoparticles (SeNPs) exposure. 57 and 47 proteins were found de-regulated by >1,5 fold in presence of selenite and SeNPs, respectively. Only 16 out of 104 proteins differentially expressed were commonly altered by selenite and SeNPs. The use of a clustered heat map allows us to visualize relations between the de-regulated proteins and exposure conditions. We identified a number of proteins involved in diverse functions and biological processes such as metabolism of carbohydrates, selenium and lipids; folding, sorting and degradation; environmental information and processing. In presence of both, selenite and SeNPs, proteins related to selenium metabolism such as cystathione beta-lyase and oxidoreductases (thioredoxine reductase and NAD/FAD oxidoreductase) were over expressed. Interestingly, the over expression of thioredoxin reductase could protect the host from oxidizing compounds. An over expression of phage proteins and chaperones with selenite was observed; this result and the fact that a lower cell count was detected when selenite was added could indicate that this latter Se species has a more deleterious effect than the nanoparticles.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
LACTIC ACID BACTERIA
dc.subject
NANOFLOW LC-MS/MS
dc.subject
SELENIUM METABOLISM
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SELENIUM NANOPARTICLES
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SILAC
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Silac-based quantitative proteomic analysis of Lactobacillus reuteri CRL 1101 response to the presence of selenite and selenium nanoparticles
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-12-11T20:20:01Z
dc.identifier.eissn
1876-7737
dc.journal.volume
195
dc.journal.pagination
53-65
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Gómez Gómez, Beatriz. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
dc.description.fil
Fil: Pérez Corona, Teresa. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
dc.description.fil
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Pescuma, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Madrid Albarrn, Yolanda. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
dc.journal.title
Journal Of Proteomics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1874391918304548
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jprot.2018.12.025
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