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Artículo

The urokinase plasminogen activator system components are regulated by vascular endothelial growth factor D in bovine oviduct

Garcia, Daniela CelesteIcon ; Russo Maenza, María AgostinaIcon ; Miceli, Dora CristinaIcon ; Valdecantos, Pablo Alberto; Roldan Olarte, Eugenia MarielaIcon
Fecha de publicación: 06/2018
Editorial: Cambridge University Press
Revista: Zygote
ISSN: 0967-1994
e-ISSN: 1469-8730
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Reproductiva

Resumen

The mammalian oviduct plays a pivotal role in the success of early reproductive events. The urokinase plasminogen activator system (uPAS) is present in the bovine oviduct and is involved in extracellular matrix remodelling through plasmin generation. This system can be regulated by several members of the vascular endothelial growth factors (VEGF) and their receptors. In this study, the VEGF-D effect on the regulation of uPAS was evaluated. First, RT-polymerase chain reaction (PCR) analyses were used to evidence the expression of VEGF-D and its receptors in oviductal epithelial cells (BOEC). VEGF-D, VEGFR2 and VEGFR3 transcripts were found in ex vivo and in vitro BOEC, while only VEGFR2 mRNA was present after in vitro conditions. VEGF-D showed a regulatory effect on uPAS gene expression in a dose-dependent manner, inducing an increase in the expression of both uPA and its receptor (uPAR) at 24 h post-induction and decreases in the expression of its inhibitor (PAI-1). In addition, the regulation of cell migration induced by VEGF-D and uPA in BOEC monolayer cultures was analyzed. The wound areas of monolayer cultures incubated with VEGF-D 10 ng/ml or uPA 10 nM were modified and significant differences were found at 24 h for both stimulations. These results indicated that uPAS and VEGF-D systems can modify the arrangement of the bovine oviductal epithelium and contribute to the correct maintenance of the oviductal microenvironment.
Palabras clave: CELL CULTURES , CELL MIGRATION , OVIDUCT , UPAS , VEGF-D
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/96427
DOI: http://dx.doi.org/10.1017/S0967199418000151
URL: https://bit.ly/2RJw65i
Colecciones
Articulos(INSIBIO)
Articulos de INST.SUP.DE INVEST.BIOLOGICAS
Citación
Garcia, Daniela Celeste; Russo Maenza, María Agostina; Miceli, Dora Cristina; Valdecantos, Pablo Alberto; Roldan Olarte, Eugenia Mariela; The urokinase plasminogen activator system components are regulated by vascular endothelial growth factor D in bovine oviduct; Cambridge University Press; Zygote; 26; 3; 6-2018; 242-249
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