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dc.contributor.author
Mulcahy, Matthew J.
dc.contributor.author
Blattman, Sydney B.
dc.contributor.author
Barrantes, Francisco Jose
dc.contributor.author
Lukas, Ronald J.
dc.contributor.author
Hawrot, Edward
dc.date.available
2020-01-30T19:49:00Z
dc.date.issued
2015-08
dc.identifier.citation
Mulcahy, Matthew J.; Blattman, Sydney B.; Barrantes, Francisco Jose; Lukas, Ronald J.; Hawrot, Edward; Resistance to inhibitors of cholinesterase 3 (Ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome; Public Library of Science; Plos One; 10; 8; 8-2015; 1-25
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/96270
dc.description.abstract
The α7-nicotinic acetylcholine receptor (α7-nAChR) is a ligand-gated ion channel widely expressed in vertebrates and is associated with numerous physiological functions. As transmembrane ion channels, α7-nAChRs need to be expressed on the surface of the plasma membrane to function. The receptor has been reported to associate with proteins involved with receptor biogenesis, modulation of receptor properties, as well as intracellular signaling cascades and some of these associated proteins may affect surface expression of α7-nAChRs. The putative chaperone resistance to inhibitors of cholinesterase 3 (Ric-3) has been reported to interact with, and enhance the surface expression of, α7-nAChRs. In this study, we identified proteins that associate with α7-nAChRs when Ric-3 is expressed. Using α-bungarotoxin (α-bgtx), we isolated and compared α7-nAChR-associated proteins from two stably transfected, human tumor-derived cell lines: SH-EP1-hα7 expressing human α7-nAChRs and the same cell line further transfected to express Ric-3, SH-EP1-hα7-Ric-3. Mass spectrometric analysis of peptides identified thirty-nine proteins that are associated with α7-nAChRs only when Ric-3 was expressed. Significantly, and consistent with reports of Ric-3 function in the literature, several of the identified proteins are involved in biological processes that may affect nAChR surface expression such as post-translational processing of proteins, protein trafficking, and protein transport. Additionally, proteins affecting the cell cycle, the cytoskeleton, stress responses, as well as cyclic AMP- and inositol triphosphate-dependent signaling cascades were identified. These results illuminate how α-bgtx may be used to isolate and identify α7-nAChRs as well as how the expression of chaperones such as Ric-3 can influence proteins associating with α7-nAChRs. These associating proteins may alter activities of α7-nAChRs to expand their functionally-relevant repertoire as well as to affect biogenesis and membrane trafficking of α7-nAChRs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
NICOTINIC ACETYLCHOLINE RECEPTOR
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PROTEOMICS
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ALPHA-7 SUBUNIT
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RIC-3
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Resistance to inhibitors of cholinesterase 3 (Ric-3) expression promotes selective protein associations with the human α7-nicotinic acetylcholine receptor interactome
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-12-27T14:03:17Z
dc.journal.volume
10
dc.journal.number
8
dc.journal.pagination
1-25
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Mulcahy, Matthew J.. University Brown; Estados Unidos
dc.description.fil
Fil: Blattman, Sydney B.. University Brown; Estados Unidos
dc.description.fil
Fil: Barrantes, Francisco Jose. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil
Fil: Lukas, Ronald J.. Barrow Neurological Institute;
dc.description.fil
Fil: Hawrot, Edward. University Brown; Estados Unidos
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0134409
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134409
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