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dc.contributor.author
Miao, Yi Liang
dc.contributor.author
Gambini, Andres
dc.contributor.author
Zhang, Yingpei
dc.contributor.author
Padilla Banks, Elizabeth
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Jefferson, Wendy N.
dc.contributor.author
Bernhardt, Miranda L.
dc.contributor.author
Huang, Weichun
dc.contributor.author
Li, Leping
dc.contributor.author
Williams, Carmen J.
dc.date.available
2020-01-29T22:23:08Z
dc.date.issued
2018-04
dc.identifier.citation
Miao, Yi Liang; Gambini, Andres; Zhang, Yingpei; Padilla Banks, Elizabeth; Jefferson, Wendy N.; et al.; Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse; Oxford University Press; Biology of Reproduction; 98; 4; 4-2018; 449-464
dc.identifier.issn
0006-3363
dc.identifier.uri
http://hdl.handle.net/11336/96205
dc.description.abstract
Understanding factors that regulate zygotic genome activation (ZGA) is critical for determining how cells are reprogrammed to become totipotent or pluripotent. There is limited information regarding how this process occurs physiologically in early mammalian embryos. Here, we identify a mediator complex subunit, MED13, as translated during mouse oocyte maturation and transcribed early from the zygotic genome. Knockdown and conditional knockout approaches demonstrate that MED13 is essential for ZGA in the mouse, in part by regulating expression of the embryo-specific chromatin remodeling complex, esBAF. The role of MED13 in ZGA is mediated in part by interactions with E2F transcription factors. In addition to MED13, its paralog, MED13L, is required for successful preimplantation embryo development. MED13L partially compensates for loss of MED13 function in preimplantation knockout embryos, but postimplantation development is not rescued by MED13L. Our data demonstrate an essential role for MED13 in supporting chromatin reprogramming and directed transcription of essential genes during ZGA.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
MED13
dc.subject
MED13L
dc.subject
MEDIATOR COMPLEX
dc.subject
OOCYTE-TO-EMBRYO TRANSITION
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PREIMPLANTATION EMBRYO
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TRANSCRIPTION
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TRANSLATION
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ZYGOTE
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Biología Reproductiva
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-01-29T19:12:16Z
dc.journal.volume
98
dc.journal.number
4
dc.journal.pagination
449-464
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Miao, Yi Liang. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Gambini, Andres. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Zhang, Yingpei. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Padilla Banks, Elizabeth. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Jefferson, Wendy N.. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Bernhardt, Miranda L.. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Huang, Weichun. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Li, Leping. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Williams, Carmen J.. National Institutes of Health; Estados Unidos
dc.journal.title
Biology of Reproduction
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/98/4/449/4794936
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/biolre/ioy004
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