Artículo
Erythropoietin attenuates LPS-induced microvascular damage in a murine model of septic acute kidney injury
Stoyanoff, Tania Romina
; Rodríguez, Juan Pablo
; Todaro, Juan Santiago; Melana Colavita, Juan Pablo
; Torres, Adriana Monica
; Aguirre, María Victoria
Fecha de publicación:
11/2018
Editorial:
Elsevier France-editions Scientifiques Medicales Elsevier
Revista:
Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
ISSN:
0753-3322
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Acute kidney injury (AKI) is a frequent complication of sepsis, with a high mortality. Hallmarks of septic-AKI include inflammation, endothelial injury, and tissue hypoxia. Therefore, it would be of interest to develop therapeutic approaches for improving the microvascular damage in septic-AKI. Erythropoietin (EPO) is a well-known cytoprotective multifunctional hormone. Thus, the aim of this study was to evaluate the protective effects of EPO on microvascular injury in a murine model of endotoxemic AKI. Male Balb/c mice were divided into four groups: control, LPS (8 mg/kg, ip.), EPO (3000 IU / kg, sc.) and LPS + EPO. A time course study (0–48 h) was designed. Experiments include, among others, immunohistochemistry and Western blottings of hypoxia-inducible transcription factor (HIF-1α), erythropoietin receptor (EPO-R), vascular endothelial growth factor system (VEGF/VEGFR-2), platelet and endothelial adhesion molecule-1 (PeCAM-1), inducible nitric oxide synthase (iNOS) and phosphorylated nuclear factor kappa B p65 (NF-κB). Data showed that EPO attenuates renal microvascular damage during septic-AKI progression through a) the decrease of HIF-1 alpha, iNOS, and NF-κB and b) the enhancement of EPO-R, PeCAM-1, VEGF, and VEGFR-2 expression. In summary, EPO renoprotection involves the attenuation of septic-induced renal hypoxia and inflammation as well as ameliorates the endotoxemic microvascular injury.
Palabras clave:
ERYTHROPOIETIN
,
HYPOXIA
,
MICROVASCULAR INJURY
,
SEPTIC AKI
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos(IQUIBA-NEA)
Articulos de INSTITUTO DE QUIMICA BASICA Y APLICADA DEL NORDESTE ARGENTINO
Articulos de INSTITUTO DE QUIMICA BASICA Y APLICADA DEL NORDESTE ARGENTINO
Citación
Stoyanoff, Tania Romina; Rodríguez, Juan Pablo; Todaro, Juan Santiago; Melana Colavita, Juan Pablo; Torres, Adriana Monica; et al.; Erythropoietin attenuates LPS-induced microvascular damage in a murine model of septic acute kidney injury; Elsevier France-editions Scientifiques Medicales Elsevier; Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie; 107; 11-2018; 1046-1055
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