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dc.contributor.author
Du, Juan
dc.contributor.author
Ma, Xin
dc.contributor.author
Shen, Bing
dc.contributor.author
Huang, Yu
dc.contributor.author
Birnbaumer, Lutz
dc.contributor.author
Yao, Xiaoqiang
dc.date.available
2020-01-28T21:00:48Z
dc.date.issued
2014-11
dc.identifier.citation
Du, Juan; Ma, Xin; Shen, Bing; Huang, Yu; Birnbaumer, Lutz; et al.; TRPV4, TRPC1, and TRPP2 assemble to form a flow-sensitive heteromeric channel; Federation of American Societies for Experimental Biology; FASEB Journal; 28; 11; 11-2014; 4677-4685
dc.identifier.issn
0892-6638
dc.identifier.uri
http://hdl.handle.net/11336/96076
dc.description.abstract
Transient receptor potential (TRP) channels, a superfamily of ion channels, can be divided into 7 subfamilies, including TRPV, TRPC, TRPP, and 4 others. Functional TRP channels are tetrameric complexes consisting of 4 pore-forming subunits. The purpose of this study was to explore the heteromerization of TRP subunits crossing different TRP subfamilies. Two-step coimmunoprecipitation (co-IP) and fluorescence resonance energy transfer (FRET) were used to determine the interaction of the different TRP subunits. Patch-clamp and cytosolic Ca2+ measurements were used to determine the functional role of the ion channels in flow conditions. The analysis demonstrated the formation of a heteromeric TRPV4-C1-P2 complex in primary cultured rat mesenteric artery endothelial cells (MAECs) and HEK293 cells that were cotransfected with TRPV4, TRPC1, and TRPP2. In functional experiments, pore-dead mutants for each of these 3 TRP isoforms nearly abolished the flow-induced cation currents and Ca2+ increase, suggesting that all 3 TRPs contribute to the ion permeation pore of the channels. We identified the first heteromeric TRP channels composed of subunits from 3 different TRP subfamilies. Functionally, this heteromeric TRPV4- C1-P2 channel mediates the flow-induced Ca2+ increase in native vascular endothelial cells.-Du, J., Ma, X., Shen, B., Huang, Y., Birnbaumer, L., Yao, X. TRPV4, TRPC1, and TRPP2 assemble to form a flowsensitive heteromeric channel.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Federation of American Societies for Experimental Biology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CA2+
dc.subject
ENDOTHELIAL CELLS
dc.subject
MESENTERIC ARTERY
dc.subject
SHEAR STRESS
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
TRPV4, TRPC1, and TRPP2 assemble to form a flow-sensitive heteromeric channel
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-28T18:26:42Z
dc.journal.volume
28
dc.journal.number
11
dc.journal.pagination
4677-4685
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Du, Juan. Chinese University Of Hong Kong; Hong Kong. Anhui Medical University; China
dc.description.fil
Fil: Ma, Xin. Chinese University Of Hong Kong; Hong Kong
dc.description.fil
Fil: Shen, Bing. Chinese University Of Hong Kong; Hong Kong. Anhui Medical University; China
dc.description.fil
Fil: Huang, Yu. Chinese University Of Hong Kong; Hong Kong
dc.description.fil
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Yao, Xiaoqiang. Chinese University Of Hong Kong; Hong Kong
dc.journal.title
FASEB Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.fasebj.org/doi/10.1096/fj.14-251652
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1096/fj.14-251652
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