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dc.contributor.author
Rabodoarivelo, Marie Sylvianne
dc.contributor.author
Brandao, A.
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Novella, M.C. Cergole
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Bombonatte, A.G.C.
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Imperiale, Belén Rocío
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Rakotosamimanana, N.
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Morcillo, Nora Susana
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Rasolofo, V.
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Palomino, Juan Carlos
dc.contributor.author
Martin, A.
dc.date.available
2020-01-28T17:47:19Z
dc.date.issued
2018-01
dc.identifier.citation
Rabodoarivelo, Marie Sylvianne; Brandao, A.; Novella, M.C. Cergole; Bombonatte, A.G.C.; Imperiale, Belén Rocío; et al.; Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study; Medknow Publications; International Journal of Mycobacteriology; 7; 1; 1-2018; 40-44
dc.identifier.issn
2212-5531
dc.identifier.uri
http://hdl.handle.net/11336/95991
dc.description.abstract
Background: In low-income countries, rapid detection of tuberculosis (TB) drug resistance is often restricted by the difficulties of transporting and storing sputum samples from remote health centers to the reference laboratories where molecular tests are available. The aim of this study was to evaluate the performance of four transport and storage systems for molecular detection of rifampicin (RIF) and isoniazid (INH) resistance. Methods: This was a multicenter study. Molecular detection of RIF and INH resistance was performed directly from smear-positive TB sputa spotted on a slide, FTA card, GenoCard, and ethanol using the Genotype MTBDRplus assay. The performance of the DNA extraction method from each storage support to detect drug resistance was assessed by calculating their sensitivity and specificity compared to the phenotypic method. Results: From all sites, the overall sensitivity and specificity for RIF-resistance detection was 88% and 85%, respectively, for slides, 86% and 92%, respectively, for GenoCard, 87% and 89%, respectively, for FTA card, and 88% and 92%, respectively, for ethanol. For INH-resistance detection, the overall sensitivity and specificity was 82% and 90%, respectively, for slides, 85% and 96%, respectively, for GenoCard, 86% and 92%, respectively, for FTA card, and 86% and 94%, respectively, for ethanol. Conclusion: Smear slides and filter cards showed to be very useful tools to facilitate DNA extraction from sputum samples with the potential to accelerate the detection of drug resistance in remote areas.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Medknow Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
FILTER CARDS
dc.subject
MOLECULAR DIAGNOSIS
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MULTIDRUG-RESISTANT DETECTION
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MYCOBACTERIUM TUBERCULOSIS
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SMEAR SLIDES
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-18T18:03:24Z
dc.identifier.eissn
2212-554X
dc.journal.volume
7
dc.journal.number
1
dc.journal.pagination
40-44
dc.journal.pais
India
dc.description.fil
Fil: Rabodoarivelo, Marie Sylvianne. Instituto Pasteur; Madagascar. Université d'Antananarivo; Madagascar
dc.description.fil
Fil: Brandao, A.. Instituto Adolfo Lutz; Brasil. Fundación Oswaldo Cruz; Brasil
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Fil: Novella, M.C. Cergole. Instituto Adolfo Lutz; Brasil
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Fil: Bombonatte, A.G.C.. Instituto Adolfo Lutz; Brasil
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Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
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Fil: Rakotosamimanana, N.. Instituto Pasteur; Madagascar
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Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
dc.description.fil
Fil: Rasolofo, V.. Instituto Pasteur; Madagascar. Université d'Antananarivo; Madagascar
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Fil: Palomino, Juan Carlos. University of Ghent; Bélgica
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Fil: Martin, A.. Université Catholique de Louvain; Bélgica
dc.journal.title
International Journal of Mycobacteriology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4103/ijmy.ijmy_193_17
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=40;epage=44;aulast=Rabodoarivelo
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