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dc.contributor.author
Coban, Ahmet Yilmaz
dc.contributor.author
Alakbarova, Gumral
dc.contributor.author
Satti, Luqman
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Tayyab, Nadia
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Zaman, Gohar
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Morcillo, Nora Susana
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Imperiale, Belén Rocío
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Wainmayer, Ingrid
dc.contributor.author
Símboli, Norberto Fabián
dc.date.available
2020-01-28T17:43:32Z
dc.date.issued
2018-10
dc.identifier.citation
Coban, Ahmet Yilmaz; Alakbarova, Gumral; Satti, Luqman; Tayyab, Nadia; Zaman, Gohar; et al.; Crystal violet decolorization assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates: A multicenter study; Medknow Publications; International Journal of Mycobacteriology; 7; 4; 10-2018; 310-314
dc.identifier.issn
2212-5531
dc.identifier.uri
http://hdl.handle.net/11336/95989
dc.description.abstract
Background: Effective control of tuberculosis is achieved by early diagnosis and drug susceptibility testing for initiation of appropriate treatment. The performance of crystal violet decolorization assay (CVDA) for susceptibility testing of Mycobacterium tuberculosis to isoniazid (INH) and rifampicin (RIF) was compared in a multicenter study. Methods: Seventy-two M. tuberculosis isolates were tested in two phases by CVDA. Results: In Phase I, the specificity, sensitivity, positive predictive value (PPV), negative predictive value (NPV), and agreement for INH were 100%, respectively. Specificity, sensitivity, PPV, NPV, and agreement for RIF were 98.2%, 100%, 94.1%, 100%, and 98.6%, respectively. In Phase II, specificity, sensitivity, PPV, NPV, and agreement were 98%, 100%, 95.4%, 100%, and 98.6% for INH, respectively. Specificity, sensitivity, PPV, NPV, and agreement for RIF were 96.3%, 88.2%, 88.2%, 96.3%, and 94.4%, respectively. Results in the study were obtained on average 10.9 ± 3.1 days in Phase I and 9.8 ± 2.2 days in Phase II. Conclusion: CVDA can be performed for drug susceptibility testing in developed and developing countries. In addition, further studies with larger sample size are needed for evaluation of this method.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Medknow Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CRYSTAL VIOLET DECOLORIZATION ASSAY
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ISONIAZID
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MULTIDRUG RESISTANT
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MYCOBACTERIUM TUBERCULOSIS
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RIFAMPICIN
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Crystal violet decolorization assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis isolates: A multicenter study
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-18T18:03:23Z
dc.identifier.eissn
2212-554X
dc.journal.volume
7
dc.journal.number
4
dc.journal.pagination
310-314
dc.journal.pais
India
dc.description.fil
Fil: Coban, Ahmet Yilmaz. Akdeniz Üniversitesi; Turquía
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Fil: Alakbarova, Gumral. Medical Microbiology Laboratory. Thalassemia Center; Azerbaiyán
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Fil: Satti, Luqman. Armed Forces Institute of Pathology; Pakistán
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Fil: Tayyab, Nadia. Armed Forces Institute of Pathology; Pakistán
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Fil: Zaman, Gohar. Armed Forces Institute of Pathology; Pakistán
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Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
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Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
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Fil: Wainmayer, Ingrid. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
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Fil: Símboli, Norberto Fabián. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
dc.journal.title
International Journal of Mycobacteriology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4103/ijmy.ijmy_141_18
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=4;spage=310;epage=314;aulast=Coban
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