Artículo
Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells
Sánchez Jiménez, Flora; Pérez Pérez, Antonio; González Yanes, Carmen; Najib, Souad; Varone, Cecilia Laura
; Sánchez Margalet, Víctor
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
01/2011
Editorial:
Elsevier Ireland
Revista:
Molecular and Cellular Endocrinology
ISSN:
0303-7207
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Leptin is produced in placenta where it has been found to be an important autocrine signal for trophoblastic growth during pregnancy, promoting antiapoptotic and trophic effects. Leptin receptor is present in trophoblastic cells and leptin may fully activate signaling. We have previously implicated the RNA-binding protein Sam68 in leptin signal transduction in immune cells. In the present work, we have studied the possible role of Sam68 in leptin receptor signaling in trophoblastic cells (JEG-3 cells). Leptin dose-dependently stimulated Sam68 phosphorylation in JEG-3 cells, as assessed by immunoprecipitation and immunoblot with anti-phosphotyrosine antibodies. As previously observed in other systems, tyrosine phosphorylation of Sam68 in response to leptin inhibits its RNA binding capacity. Besides, leptin stimulation dose-dependently increases Sam68 expression in JEG-3 cells, as assessed by quantitative PCR. Consistently, the amount of Sam68 protein is increased after 24. h of leptin stimulation of trophoblastic cells. In order to study the possible role of Sam68 on leptin receptor synthesis, we employed antisense strategy to knockdown the expression of Sam68. We have found that a decrease in Sam68 expression leads to a decrease in leptin receptor amount in JEG-3 cells, as assessed both by quantitative PCR and immunoblot.These results strongly suggest the participation of Sam68 in leptin receptor signaling in human trophoblastic cells, and therefore, Sam68 may mediate some of the leptin effects in placenta.
Palabras clave:
LEPTIN RECEPTOR
,
PLACENTA
,
SAM68
,
SIGNAL TRANSDUCTION
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Sánchez Jiménez, Flora; Pérez Pérez, Antonio; González Yanes, Carmen; Najib, Souad; Varone, Cecilia Laura; et al.; Leptin receptor activation increases Sam68 tyrosine phosphorylation and expression in human trophoblastic cells; Elsevier Ireland; Molecular and Cellular Endocrinology; 332; 1-2; 1-2011; 221-227
Compartir
Altmétricas