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dc.contributor.author
Rodero, Camila Fernanda
dc.contributor.author
Fioramonti Calixto, Giovana Maria
dc.contributor.author
dos Santos, Karen Cristina
dc.contributor.author
Sato, Mariana Rillo
dc.contributor.author
Aparecido dos Santos Ramos, Matheus
dc.contributor.author
Miró, María Soledad
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Rodriguez, Emilse
dc.contributor.author
Vigezzi, Cecilia
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Bauab, Tais Maria
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Sotomayor, Claudia Elena
dc.contributor.author
Chorilli, Marlus
dc.date.available
2020-01-27T18:02:28Z
dc.date.issued
2018-10
dc.identifier.citation
Rodero, Camila Fernanda; Fioramonti Calixto, Giovana Maria; dos Santos, Karen Cristina; Sato, Mariana Rillo; Aparecido dos Santos Ramos, Matheus; et al.; Curcumin-Loaded Liquid Crystalline Systems for Controlled Drug Release and Improved Treatment of Vulvovaginal Candidiasis; American Chemical Society; Molecular Pharmaceutics; 15; 10; 10-2018; 4491-4504
dc.identifier.issn
1543-8384
dc.identifier.uri
http://hdl.handle.net/11336/95851
dc.description.abstract
Vulvovaginal candidiasis (VVC) is the most common infection caused by Candida albicans and greatly reduces the quality of life of women affected by it. Due to the ineffectiveness of conventional treatments, there is growing interest in research involving compounds of natural origin. One such compound is curcumin (CUR), which has been proven to be effective against this microorganism. However, some of CUR's physicochemical properties, especially its low aqueous solubility, make the therapeutic application of this compound difficult. Thus, the incorporation of CUR in mucoadhesive liquid crystalline systems (MLCSs) for vaginal administration may be an efficient strategy for the treatment of VVC. MLCSs are capable of potentiating the compound's action, releasing it in a controlled manner, and can enable longer exposure at the site of infection. In this study, MLCSs consisting of oleic acid and ergosterol 5:1 (w/w) as the oily phase, PPG-5-CETETH-20 as the surfactant, and a polymer dispersion of 1% chitosan as the aqueous phase, were developed for the application of CUR (MLCS-CUR) in VVC treatment. The formulations were characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), oscillatory rheometry, continuous shear rheometry, texture profile analysis, and in vitro mucoadhesion. In addition, the antimicrobial activity was evaluated in vitro, and the effects on local fungal burden and cytokine profiles were investigated in a murine model of VVC. PLM and SAXS showed that the developed formulations presented a characteristic of a microemulsion. However, after the addition of artificial vaginal mucus (AVM), PLM showed that the formulations had structures similar to the "Maltese cross" characteristic of lamellar MLCS. Mucoadhesive test results showed an increase in the mucoadhesive strength of these formulations. Rheology analyses suggested long-lasting action of the formulation at the infected site. The in vitro antimicrobial activity assays suggested that CUR possesses antifungal activity against Candida albicans, determined after its incorporation into the MLCS. Further, MLCS-CUR was also more effective in vivo in the control of vaginal infection than treatment with fluconazole. Immunological assays showed that the ratio of pro-inflammatory (IL-1β) to anti-inflammatory (TGF-β) cytokines has decreased and that there is a reduction in the number of polymorphonuclear neutrophils recruited to the vaginal lumen, showing that treatment with MLCS-CUR was effective in modulating the inflammatory reaction associated with the infection. The results suggest that MLCSs could potentially be used in the treatment of VVC with CUR.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CANDIDA ALBICANS
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CURCUMIN
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MUCOADHESIVE LIQUID CRYSTALLINE SYSTEMS
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VULVOVAGINAL CANDIDIASIS
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Micología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Curcumin-Loaded Liquid Crystalline Systems for Controlled Drug Release and Improved Treatment of Vulvovaginal Candidiasis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-22T16:35:02Z
dc.journal.volume
15
dc.journal.number
10
dc.journal.pagination
4491-4504
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington DC
dc.description.fil
Fil: Rodero, Camila Fernanda. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
dc.description.fil
Fil: Fioramonti Calixto, Giovana Maria. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
dc.description.fil
Fil: dos Santos, Karen Cristina. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
dc.description.fil
Fil: Sato, Mariana Rillo. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
dc.description.fil
Fil: Aparecido dos Santos Ramos, Matheus. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
dc.description.fil
Fil: Miró, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil
Fil: Rodriguez, Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil
Fil: Vigezzi, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil
Fil: Bauab, Tais Maria. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
dc.description.fil
Fil: Sotomayor, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.description.fil
Fil: Chorilli, Marlus. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
dc.journal.title
Molecular Pharmaceutics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1021/acs.molpharmaceut.8b00507
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.8b00507
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