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dc.contributor.author
Anton, Carlos
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dc.contributor.author
Valdez, Javier Esteban
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dc.contributor.author
Roncero, Cesar
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dc.date.available
2020-01-24T23:03:58Z
dc.date.issued
2018-04
dc.identifier.citation
Anton, Carlos; Valdez, Javier Esteban; Roncero, Cesar; The functional specialization of exomer as a cargo adaptor during the evolution of fungi; Genetics Society of America; Genetics; 208; 4; 4-2018; 1483-1498
dc.identifier.issn
0016-6731
dc.identifier.uri
http://hdl.handle.net/11336/95822
dc.description.abstract
Yeast exomer is a heterotetrameric complex that is assembled at the trans-Golgi network, which is required for the delivery of a distinct set of proteins to the plasma membrane using ChAPs (Chs5-Arf1 binding proteins) Chs6 and Bch2 as dedicated cargo adaptors. However, our results show a significant functional divergence between them, suggesting an evolutionary specialization among the ChAPs. Moreover, the characterization of exomer mutants in several fungi indicates that exomer’s function as a cargo adaptor is a late evolutionary acquisition associated with several gene duplications of the fungal ChAPs ancestor. Initial gene duplication led to the formation of the two ChAPs families, Chs6 and Bch1, in the Saccaromycotina group, which have remained functionally redundant based on the characterization of Kluyveromyces lactis mutants. The whole-genome duplication that occurred within the Saccharomyces genus facilitated a further divergence, which allowed Chs6/Bch2 and Bch1/Bud7 pairs to become specialized for specific cellular functions. We also show that the behavior of S. cerevisiae Chs3 as an exomer cargo is associated with the presence of specific cytosolic domains in this protein, which favor its interaction with exomer and AP-1 complexes. However, these domains are not conserved in the Chs3 proteins of other fungi, suggesting that they arose late in the evolution of fungi associated with the specialization of ChAPs as cargo adaptors.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Genetics Society of America
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
EVOLUTION
dc.subject
EXOMER
dc.subject
INTRACELLULAR TRAFFIC
dc.subject
YEAST
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The functional specialization of exomer as a cargo adaptor during the evolution of fungi
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-22T16:43:31Z
dc.journal.volume
208
dc.journal.number
4
dc.journal.pagination
1483-1498
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Anton, Carlos. Consejo Superior de Investigaciones Científicas; España. Universidad de Salamanca; España
dc.description.fil
Fil: Valdez, Javier Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
dc.description.fil
Fil: Roncero, Cesar. Consejo Superior de Investigaciones Científicas; España. Universidad de Salamanca; España
dc.journal.title
Genetics
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1534/genetics.118.300767
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