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dc.contributor.author
Filgueira, Marcelo R.  
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Huang, Yuan  
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Witt, Klaus  
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Castells, Cecilia Beatriz Marta  
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Carr, Peter W.  
dc.date.available
2020-01-21T21:44:24Z  
dc.date.issued
2011-12  
dc.identifier.citation
Filgueira, Marcelo R.; Huang, Yuan; Witt, Klaus; Castells, Cecilia Beatriz Marta; Carr, Peter W.; Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting; American Chemical Society; Analytical Chemistry; 83; 24; 12-2011; 9531-9539  
dc.identifier.issn
0003-2700  
dc.identifier.uri
http://hdl.handle.net/11336/95499  
dc.description.abstract
The use of flow splitters between the two dimensions in online comprehensive two-dimensional (2D) liquid chromatography (LC × LC) has not received very much attention, in comparison with their use in 2D gas chromatography (GC × GC), where they are quite common. In principle, splitting the flow after the first dimension column and performing online LC × LC on this constant fraction of the first dimension effluent should allow the two dimensions to be optimized almost independently. When there is no flow splitting, any change in the first-dimension flow rate has an immediate impact on the second dimension. With a flow splitter, one could, for example, double the flow rate into the first dimension column and perform a 1:1 flow split without changing the sample loop size or the sampler's collection time. Of course, the sensitivity would be diminished, but this can be partially compensated through the use of a larger injection; this will likely only amount to a small price to pay for this increased resolving power and system flexibility. Among other benefits, we found a 2-fold increase in the corrected 2D peak capacity and the number of observed peaks for a 15-min analysis time, using a post-first-dimension flow splitter. At a fixed analysis time, this improvement results primarily from an increase in the gradient time, resulting from the reduced system re-equilibration time, and, to a smaller extent, it is due to the increased peak capacity achieved by full optimization of the first dimension.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
American Chemical Society  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Two dimensional LC  
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Peak capacity  
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Fast Gradient  
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Flow Splitting  
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Química Analítica  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-01-21T18:49:24Z  
dc.journal.volume
83  
dc.journal.number
24  
dc.journal.pagination
9531-9539  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Washington  
dc.description.fil
Fil: Filgueira, Marcelo R.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. University of Minnesota; Estados Unidos  
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Fil: Huang, Yuan. University of Minnesota; Estados Unidos  
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Fil: Witt, Klaus. Agilent Technologies Deutschland; Alemania  
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Fil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina  
dc.description.fil
Fil: Carr, Peter W.. University of Minnesota; Estados Unidos  
dc.journal.title
Analytical Chemistry  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/ac202317m  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/ac202317m