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dc.contributor.author
Gagliardi, Leonardo Gabriel  
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Castells, Cecilia Beatriz Marta  
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Ràfols, Clara  
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Rosés, Martí  
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Bosch, Elisabeth  
dc.date.available
2020-01-21T19:53:10Z  
dc.date.issued
2008-04  
dc.identifier.citation
Gagliardi, Leonardo Gabriel; Castells, Cecilia Beatriz Marta; Ràfols, Clara; Rosés, Martí; Bosch, Elisabeth; Effect of temperature on the chromatographic retention of ionizable compounds. III. Modeling retention of Pharmaceuticals as a function of eluent pH and column temperature in RPLC; Wiley VCH Verlag; Journal of Separation Science; 31; 6-7; 4-2008; 969-980  
dc.identifier.issn
1615-9306  
dc.identifier.uri
http://hdl.handle.net/11336/95445  
dc.description.abstract
We propose a general simple equation for accurately predicting the retention factors of ionizable compounds upon simultaneous changes in mobile phase pH and column temperature at a given hydroorganic solvent composition. Only four independent experiments provide the input data: retention factors measured in two pH buffered mobile phases at extreme acidic and basic pH values (e.g., at least ±2 pH units far from the analyte pKa) and at two column temperatures. The equations, derived from the basic thermodynamics of the acid-base equilibria, additionally require the knowledge of the solute pKa and enthalpies of acid - base dissociation of both the solute and the buffer components in the hydroorganic solvent mixture. The performance of the predictive model is corroborated with the comparison between theoretical and experimental retention factors of several weak acids and bases of important pharmacological activity, in mobile phases containing different buffer solutions prepared in 25% w/w ACN in water and at several temperatures.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley VCH Verlag  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
COLUMN TEMPERATURE  
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IONIZABLE SOLUTES  
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PREDICTIVE MODEL  
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PROFENS, Β-BLOCKERS, ALKALOIDS  
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Química Analítica  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Effect of temperature on the chromatographic retention of ionizable compounds. III. Modeling retention of Pharmaceuticals as a function of eluent pH and column temperature in RPLC  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2020-01-21T18:44:48Z  
dc.journal.volume
31  
dc.journal.number
6-7  
dc.journal.pagination
969-980  
dc.journal.pais
Alemania  
dc.journal.ciudad
Weinheim  
dc.description.fil
Fil: Gagliardi, Leonardo Gabriel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina  
dc.description.fil
Fil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
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Fil: Ràfols, Clara. Universidad de Barcelona; España  
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Fil: Rosés, Martí. Universidad de Barcelona; España  
dc.description.fil
Fil: Bosch, Elisabeth. Universidad de Barcelona; España  
dc.journal.title
Journal of Separation Science  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jssc.200700491/abstract  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jssc.200700491