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Artículo

Sudestada1, a Drosophila ribosomal prolyl-hydroxylase required for mRNA translation, cell homeostasis, and organ growth

Katz, Maximiliano JavierIcon ; Acevedo, Julieta MariaIcon ; Loenarz, Christoph; Galagovsky, DiegoIcon ; Liu Yi, Phebee; Pérez, MarceloIcon ; Thalhammer, Armin; Sekirnik, Rok; Ge, Wei; Melani, MarianaIcon ; Thomas, Maria GabrielaIcon ; Simonetta, Sergio HernanIcon ; Boccaccio, Graciela LidiaIcon ; Schofield, Christoper J; Cockman, Matthew E; Ratcliffe, Peter J; Wappner, PabloIcon
Fecha de publicación: 03/2014
Editorial: National Academy Of Sciences
Revista: Proceedings Of The National Academy Of Sciences Of The United States Of America
ISSN: 0027-8424
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Genome sequences predict the presence of many 2-oxoglutarate (2OG)-dependent oxygenases of unknown biochemical and biological functions in Drosophila. Ribosomal protein hydroxylation is emerging as an important 2OG oxygenase catalyzed pathway, but its biological functions are unclear. We report investigations on the function of Sudestada1 (Sud1), a Drosophila ribosomal oxygenase. As with its human and yeast homologs, OGFOD1 and Tpa1p, respectively, we identified Sud1 to catalyze prolyl-hydroxylation of the small ribosomal subunit protein RPS23. Like OGFOD1, Sud1 catalyzes a single prolyl-hydroxylation of RPS23 in contrast to yeast Tpa1p, where Pro-64 dihydroxylation is observed. RNAi-mediated Sud1 knockdown hinders normal growth in different Drosophila tissues. Growth impairment originates from both reduction of cell size and diminution of the number of cells and correlates with impaired translation efficiency and activation of the unfolded protein response in the endoplasmic reticulum. This is accompanied by phosphorylation of eIF2α and concomitant formation of stress granules, as well as promotion of autophagy and apoptosis. These observations, together with those on enzyme homologs described in the companion articles, reveal conserved biochemical and biological roles for a widely distributed ribosomal oxygenase.
Palabras clave: Sudestada , Drosophila , Mrna , Prolyl Hidroxilase
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/9544
URL: http://www.pnas.org/content/111/11/4025.long
DOI: http://dx.doi.org/10.1073/pnas.1314485111
Colecciones
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Citación
Katz, Maximiliano Javier; Acevedo, Julieta Maria; Loenarz, Christoph; Galagovsky, Diego; Liu Yi, Phebee; et al.; Sudestada1, a Drosophila ribosomal prolyl-hydroxylase required for mRNA translation, cell homeostasis, and organ growth; National Academy Of Sciences; Proceedings Of The National Academy Of Sciences Of The United States Of America; 111; 11; 3-2014; 4025-4030
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