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Artículo

Automated method for the total creatinine determination in dehydrated broths

Acebal, Carolina CeciliaIcon ; Centurión, María Eugenia; Lista, Adriana Guillermina; Fernández Band, Beatriz SusanaIcon
Fecha de publicación: 01/2006
Editorial: Taylor & Francis
Revista: Analytical Letters
ISSN: 0003-2719
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Química Analítica

Resumen

In order to improve the quality control of dehydrated broth, a new automated method was developed to determine total creatinine in dehydrated broths. The sample pretreatment was coupled on-line with the Flow Injection Analysis (FIA) system for analyte determination by the classical Jaffe reaction, stopped flow methodology, and spectrophotometric detection. The time consumed was reduced from 7h, which is necessary with the official method, to 25 min. The calibration graph is linear between 0.342-1.368 mg creatinine/ 100 mL. The relative standard deviation (RSD%) was 1.7%, the sample throughput was 7 h -1, and the detection limit was 0.185 mg creatinine/100 mL. The validation of the proposed method was carried out with real samples. The obtained results were compared with those obtained from the Association of Official Analytical Chemists (AOAC) reference method.
Palabras clave: AUTOMATED ANALYSIS , CREATININE , FIA , MEAT PRODUCT
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/95350
DOI: https://doi.org/10.1080/00032710500477175
URL: https://www.tandfonline.com/doi/abs/10.1080/00032710500477175
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Articulos(INQUISUR)
Articulos de INST.DE QUIMICA DEL SUR
Citación
Acebal, Carolina Cecilia; Centurión, María Eugenia; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Automated method for the total creatinine determination in dehydrated broths; Taylor & Francis; Analytical Letters; 39; 2; 1-2006; 387-394
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