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dc.contributor.author
Golemba, Marcelo Darío
dc.contributor.author
Parreño, Gladys Viviana
dc.contributor.author
Jones, Leandro Roberto
dc.date.available
2020-01-16T18:22:33Z
dc.date.issued
2008-06
dc.identifier.citation
Golemba, Marcelo Darío; Parreño, Gladys Viviana; Jones, Leandro Roberto; Simple procedures to obtain exogenous internal controls for use in RT-PCR detection of bovine pestiviruses; Academic Press Ltd - Elsevier Science Ltd; Molecular And Cellular Probes; 22; 3; 6-2008; 212-214
dc.identifier.issn
0890-8508
dc.identifier.uri
http://hdl.handle.net/11336/94912
dc.description.abstract
Pestiviruses are ubiquitous pathogens of cattle and frequent adventitious viruses in biologicals. Furthermore, it has been suggested that these agents might be related to infantile gastroenteritis and microencephaly. Since the virus is highly prevalent in fetal bovine serum, the risk of contamination is high in most laboratories. Thus, the implementation of detection methods in all laboratories is of worth. Despite continuous surveillance, these agents have been detected in cell lines, fetal bovine serum, live and inactivated animal and human vaccines and interferon for human use. In this report, DNA and RNA internal controls (ICs) which can be implemented in laboratories with minimal equipment are described. The developed standards can be added before RNA purification, allowing to monitor all steps of the protocol (viral RNA extraction, reverse transcription and cDNA amplification). It is shown that inhibitory effects that could lead to decreased sensitivity can be minimized by controlling the amount of mimic molecules added to the samples. A method to avoid the problem of DNA traces present in in vitro transcribed RNA preparations is provided.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Ltd - Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOLOGICALS
dc.subject
BVDV
dc.subject
ENDOGENOUS
dc.subject
INTERNAL CONTROL
dc.subject
PCR
dc.subject
PESTIVIRUS
dc.subject.classification
Ciencias de las Plantas, Botánica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Simple procedures to obtain exogenous internal controls for use in RT-PCR detection of bovine pestiviruses
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-12-12T14:50:42Z
dc.journal.volume
22
dc.journal.number
3
dc.journal.pagination
212-214
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Golemba, Marcelo Darío. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Jones, Leandro Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.journal.title
Molecular And Cellular Probes
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0890850808000170
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.mcp.2007.12.003
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