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dc.contributor.author
Martin, Stella C.
dc.contributor.author
Steiger, Janine L.
dc.contributor.author
Gravielle, Maria Clara
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dc.contributor.author
Lyons, Helen R.
dc.contributor.author
Russek, Shelley J.
dc.contributor.author
Farb, David H.
dc.date.available
2020-01-15T15:15:24Z
dc.date.issued
2004-05
dc.identifier.citation
Martin, Stella C.; Steiger, Janine L.; Gravielle, Maria Clara; Lyons, Helen R.; Russek, Shelley J.; et al.; Differential expression of γ‐aminobutyric acid type B receptor subunit mRNAs in the developing nervous system and receptor coupling to adenylyl cyclase in embryonic neurons; Wiley-liss, Div John Wiley & Sons Inc; Journal Of Comparative Neurology; 473; 1; 5-2004; 16-29
dc.identifier.issn
0021-9967
dc.identifier.uri
http://hdl.handle.net/11336/94734
dc.description.abstract
γ‐Aminobutyric acid type B receptors (GABABRs) mediate both slow inhibitory synaptic activity in the adult nervous system and motility signals for migrating embryonic cortical cells. Previous papers have described the expression of GABABRs in the adult brain, but the expression and functional significance of these gene products in the embryo are largely unknown. Here we examine GABABR expression from rat embryonic day 10 (E10) to E18 compared with adult and ask whether embryonic cortical neurons contain functional GABABR. GABABR1 transcript levels greatly exceed GABABR2 levels in the developing neural tube at E11, and olfactory bulb and striatum at E17 but equalize in most regions of adult nervous tissue, except for the glomerular and granule cell layers of the main olfactory bulb and the striatum. Consistent with expression differences, the binding affinity of GABA for GABABRs is significantly lower in adult striatum compared with cerebellum. Multiple lines of evidence from in situ hybridization, RNase protection, and real‐time PCR demonstrate that GABABR1a, GABABR1b, GABABR1h (a subunit subtype, lacking a sushi domain, that we have identified in embryonic rat brain), GABABR2, and GABABL transcript levels are not coordinately regulated. Despite the functional requirement for a heterodimer of GABABR subunits, the expression of each subunit mRNA is under independent control during embryonic development, and, by E18, GABABRs are negatively coupled to adenylyl cyclase in neocortical neurons. The presence of embryonic GABABR transcripts and protein and functional receptor coupling indicates potentially important roles for GABABRs in modulation of synaptic transmission in the developing embryonic nervous system.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-liss, Div John Wiley & Sons Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
EMBRYO
dc.subject
GABA
dc.subject
IN SITU HYBRIDIZATION
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OLFACTORY BULB
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RECEPTOR
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STRIATUM
dc.subject.classification
Otros Tópicos Biológicos
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Differential expression of γ‐aminobutyric acid type B receptor subunit mRNAs in the developing nervous system and receptor coupling to adenylyl cyclase in embryonic neurons
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-11-25T17:47:09Z
dc.journal.volume
473
dc.journal.number
1
dc.journal.pagination
16-29
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Nueva York
dc.description.fil
Fil: stella C. MARTIN. Laboratory of Molecular Neurobiology, Department of Pharmacology, Boston University
School of Medicine, Boston, Massachusetts; Estados Unidos
dc.description.fil
Fil: Russek, Shelley June. Laboratory of Molecular Neurobiology, Department of Pharmacology, Boston University
School of Medicine, Boston, Massachusetts; Argentina
dc.description.fil
Fil: Farb, David H.. Laboratory of Molecular Neurobiology, Department of Pharmacology, Boston University
School of Medicine, Boston, Massachusetts; Estados Unidos
dc.description.fil
Fil: Maria Clara Gravielle. Laboratory of Molecular Neurobiology, Department of Pharmacology, Boston University
School of Medicine, Boston, Massachusetts; Estados Unidos
dc.journal.title
Journal Of Comparative Neurology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.20094
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/cne.20094
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