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dc.contributor.author
Trevani, Analía Silvina
dc.contributor.author
Chorny, Alejo
dc.contributor.author
Salamone, Gabriela Veronica
dc.contributor.author
Vermeulen, Elba Monica
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Gamberale, Romina
dc.contributor.author
Schettini, Jorge
dc.contributor.author
Raiden, Silvina Claudia
dc.contributor.author
Geffner, Jorge Raúl
dc.date.available
2020-01-14T18:26:51Z
dc.date.issued
2003-11
dc.identifier.citation
Trevani, Analía Silvina; Chorny, Alejo; Salamone, Gabriela Veronica; Vermeulen, Elba Monica; Gamberale, Romina; et al.; Bacterial DNA activates human neutrophils by a CpG‐independent pathway; Wiley VCH Verlag; European Journal of Immunology; 33; 11; 11-2003; 3164-3174
dc.identifier.issn
0014-2980
dc.identifier.uri
http://hdl.handle.net/11336/94630
dc.description.abstract
Bacterial DNA stimulates macrophages, monocytes, B lymphocytes, NK cells, and dendritic cells in a CpG‐dependent manner. In this work we demonstrate that bacterial DNA, but not mammalian DNA, induces human neutrophil activation as assessed by L‐selectin shedding, CD11b upregulation, and stimulation of cellular shape change, IL‐8 secretion, and cell migration. Induction of these responsesis not dependent on the presence of unmethylated CpG motifs, as neutrophil stimulatory properties were neither modified by CpG‐methylation of bacterial DNA nor reproduced by oligonucleotides bearing CpG motifs. We found that human neutrophils express Toll‐like receptor (TLR) 9 mRNA. However, as expected for a CpG‐independent mechanism, activation does not involve a TLR9‐dependent signaling pathway; neutrophil stimulation was not prevented by immobilization of bacterial DNA or by wortmannin or chloroquine, two agents that inhibit TLR9 signaling. Of note, both single‐stranded and double‐stranded DNA were able to induce activation, suggesting that neutrophils might be activated by bacterial DNA at inflammatory foci even in the absence of conditions required to induce DNA denaturation. Our findings provide the first evidence that neutrophils might be alerted to the presence of invading bacteria through recognition of its DNA via a novel mechanism not involving CpG motifs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley VCH Verlag
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BACTERIAL DNA
dc.subject
CPG MOTIF
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NEUTROPHIL
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OLIGODEOXYNUCLEOTIDE
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TLR9
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Bacterial DNA activates human neutrophils by a CpG‐independent pathway
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-10T19:32:10Z
dc.journal.volume
33
dc.journal.number
11
dc.journal.pagination
3164-3174
dc.journal.pais
Alemania
dc.journal.ciudad
Weinheim
dc.journal.title
European Journal of Immunology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/eji.200324334
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/eji.200324334


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