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dc.contributor.author
Kalinka, Julia  
dc.contributor.author
Hachmeister, Marie  
dc.contributor.author
Geraci, Jennifer  
dc.contributor.author
Sordelli, Daniel Oscar  
dc.contributor.author
Hansen, Uwe  
dc.contributor.author
Niemann, Silke  
dc.contributor.author
Oetermann, Sylvia  
dc.contributor.author
Peters, Georg  
dc.contributor.author
Löffler, Bettina  
dc.contributor.author
Tuchscherr, Lorena Paola Nelly  
dc.date.available
2020-01-13T14:24:19Z  
dc.date.issued
2014-11  
dc.identifier.citation
Kalinka, Julia; Hachmeister, Marie; Geraci, Jennifer; Sordelli, Daniel Oscar; Hansen, Uwe; et al.; Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation; Elsevier Gmbh; International Journal of Medical Microbiology (print); 304; 8; 11-2014; 1038-1049  
dc.identifier.issn
1438-4221  
dc.identifier.uri
http://hdl.handle.net/11336/94483  
dc.description.abstract
Osteomyelitis is a severe inflammatory disease of the bone that is mainly caused by Staphylococcus aureus. Particularly, bone infections are difficult to treat and can develop into a chronic course with a high relapsing rate despite of antimicrobial treatments. The complex interaction of staphylococci with osseous tissue and the bacterial ability to invade host cells are thought to determine the severity of infection. Yet, defined bacterial virulence factors responsible for the pathogenesis of osteomyelitis have not been clearly identified. The aim of this study was to detect S. aureus virulence factors that are associated with osteomyelitis and contribute to a chronic course of infection. To this purpose, we collected 41 S. aureus isolates, each 11 from acute osteomyelitis (infection period less than 2 months), 10 from chronic osteomyelitis (infection period more than 12 months), 10 from sepsis and 10 from nasal colonization. All isolates were analyzed for gene expression and in functional in-vitro systems. Adhesion assays to bone matrix revealed that all isolates equally bound to matrix structures, but invasion assays in human osteoblasts showed a high invasive capacity of chronic osteomyelitis isolates. The high invasion rate could not be explained by defined adhesins, as all infecting strains expressed a multitude of adhesins that act together and determine the level of adhesion. Following host cell invasion isolates from chronic osteomyelitis induced less cytotoxicity than all other isolates and a higher percentage of Small-colony-variant (SCV)-formation, which represents an adaptation mechanism during long-term persistence. Isolates from acute and chronic osteomyelitis strongly produced biofilm and highly expressed agr and sarA that regulate secreted virulence factors and induced an inflammatory response in osteoblasts. In conclusion, chronic osteomyelitis isolates were characterized by a high host cell invasion rate, low cytotoxicity and the ability to persist and adapt within osteoblasts. Furthermore, isolates from both acute and chronic osteomyelitis strongly produced biofilm and induced high levels of host cell inflammation, which may explain tissue destruction and bone deformation observed as typical complications of long-lasting bone infections.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Gmbh  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
ADHESINS  
dc.subject
CHRONIC INFECTIONS  
dc.subject
HOST CELL INVASION  
dc.subject
INTRACELLULAR PERSISTENCE  
dc.subject
S. AUREUS OSTEOMYELITIS  
dc.subject.classification
Inmunología  
dc.subject.classification
Medicina Básica  
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Staphylococcus aureus isolates from chronic osteomyelitis are characterized by high host cell invasion and intracellular adaptation, but still induce inflammation  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-12-27T14:06:45Z  
dc.journal.volume
304  
dc.journal.number
8  
dc.journal.pagination
1038-1049  
dc.journal.pais
Alemania  
dc.description.fil
Fil: Kalinka, Julia. University Hospital of Münster; Alemania  
dc.description.fil
Fil: Hachmeister, Marie. University Hospital of Münster; Alemania  
dc.description.fil
Fil: Geraci, Jennifer. University Hospital of Münster; Alemania. Jena University Hospital; Alemania  
dc.description.fil
Fil: Sordelli, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología; Argentina  
dc.description.fil
Fil: Hansen, Uwe. University Hospital of Münster; Alemania  
dc.description.fil
Fil: Niemann, Silke. University Hospital of Münster; Alemania  
dc.description.fil
Fil: Oetermann, Sylvia. University Hospital of Münster; Alemania  
dc.description.fil
Fil: Peters, Georg. University Hospital of Münster; Alemania  
dc.description.fil
Fil: Löffler, Bettina. University Hospital of Münster; Alemania. Jena University Hospital; Alemania  
dc.description.fil
Fil: Tuchscherr, Lorena Paola Nelly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University Hospital of Münster; Alemania. Jena University Hospital; Alemania  
dc.journal.title
International Journal of Medical Microbiology (print)  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1438422114000952  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ijmm.2014.07.013