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dc.contributor.author
Sun, Jin  
dc.contributor.author
Zhang, Huoming  
dc.contributor.author
Wang, Hao  
dc.contributor.author
Heras, Horacio  
dc.contributor.author
Dreon, Marcos Sebastian  
dc.contributor.author
Ituarte, Santiago  
dc.contributor.author
Ravasi, Timothy  
dc.contributor.author
Qian, Pei-Yuan  
dc.contributor.author
Qiu, Jian-Wen  
dc.date.available
2020-01-10T21:08:48Z  
dc.date.issued
2012-08  
dc.identifier.citation
Sun, Jin; Zhang, Huoming; Wang, Hao; Heras, Horacio; Dreon, Marcos Sebastian; et al.; First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition; American Chemical Society; Journal of Proteome Research; 11; 8; 8-2012; 4240-4248  
dc.identifier.issn
1535-3893  
dc.identifier.uri
http://hdl.handle.net/11336/94414  
dc.description.abstract
Pomacea canaliculata is a freshwater snail that deposits eggs on solid substrates above the water surface. Previous studies have emphasized the nutritional and protective functions of the three most abundant perivitelline fluid (PVF) protein complexes (ovorubin, PV2, and PV3) during its embryonic development, but little is known about the structure and function of other less abundant proteins. Using 2-DE, SDS-PAGE, MALDI TOF/TOF, and LC-MS/MS, we identified 59 proteins from the PVF of P. canaliculata, among which 19 are novel. KEGG analysis showed that the functions of the majority of these proteins are "unknown" (n = 34), "environmental information processing" (10), 9 of which are related to innate immunity, and "metabolism" (7). Suppressive subtractive hybridization revealed 21 PVF genes to be specific to the albumen gland, indicating this organ is the origin of many of the PVF proteins. Further, the 3 ovorubin subunits were identified with 30.2-35.0% identity among them, indicating their common origin but ancient duplications. Characterization of the PVF proteome has opened the gate for further studies aiming to understand the evolution of the novel proteins and their contribution to the switch to aerial oviposition.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
American Chemical Society  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
APPLE SNAIL  
dc.subject
NOVEL PROTEIN  
dc.subject
PERIVITELLINE FLUID  
dc.subject
POMACEA CANALICULATA  
dc.subject
PROTEOMICS  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
First proteome of the egg perivitelline fluid of a freshwater gastropod with aerial oviposition  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-05-03T17:36:20Z  
dc.journal.volume
11  
dc.journal.number
8  
dc.journal.pagination
4240-4248  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Washington  
dc.description.fil
Fil: Sun, Jin. Hong Kong Baptist University; Hong Kong  
dc.description.fil
Fil: Zhang, Huoming. Hong Kong Baptist University; Hong Kong  
dc.description.fil
Fil: Wang, Hao. Hong Kong University Of Science And Technology; Hong Kong  
dc.description.fil
Fil: Heras, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina  
dc.description.fil
Fil: Dreon, Marcos Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina  
dc.description.fil
Fil: Ituarte, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina  
dc.description.fil
Fil: Ravasi, Timothy. King Abdullah University Of Science And Technology; Arabia Saudita  
dc.description.fil
Fil: Qian, Pei-Yuan. Hong Kong University Of Science And Technology; Hong Kong  
dc.description.fil
Fil: Qiu, Jian-Wen. Hong Kong Baptist University; Hong Kong  
dc.journal.title
Journal of Proteome Research  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/pr3003613  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/pr3003613