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dc.contributor.author
Bustos Sanmamed, Maria del Pilar
dc.contributor.author
Hudik, Elodie
dc.contributor.author
Laffont, Carole
dc.contributor.author
Reynes, Christelle
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Sallet, Erika
dc.contributor.author
Wen, Jiangqi
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Mysore, Kirankumar S.
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Camproux, Anne Claude
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Hartmann, Caroline
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Gouzy, Jérome
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Frugier, Florian
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Crespi, Martin
dc.contributor.author
Lelandais Brière, Christine
dc.date.available
2020-01-09T21:23:47Z
dc.date.issued
2014-12
dc.identifier.citation
Bustos Sanmamed, Maria del Pilar; Hudik, Elodie; Laffont, Carole; Reynes, Christelle; Sallet, Erika; et al.; A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development; Wiley Blackwell Publishing, Inc; Plant Biotechnology Journal; 12; 9; 12-2014; 1308-1318
dc.identifier.issn
1467-7644
dc.identifier.uri
http://hdl.handle.net/11336/94271
dc.description.abstract
Summary: RNA-dependent RNA polymerase 6 (RDR6) and suppressor of gene silencing 3 (SGS3) act together in post-transcriptional transgene silencing mediated by small interfering RNAs (siRNAs) and in biogenesis of various endogenous siRNAs including the tasiARFs, known regulators of auxin responses and plant development. Legumes, the third major crop family worldwide, has been widely improved through transgenic approaches. Here, we isolated rdr6 and sgs3 mutants in the model legume Medicago truncatula. Two sgs3 and one rdr6 alleles led to strong developmental defects and impaired biogenesis of tasiARFs. In contrast, the rdr6.1 homozygous plants produced sufficient amounts of tasiARFs to ensure proper development. High throughput sequencing of small RNAs from this specific mutant identified 354 potential MtRDR6 substrates, for which siRNA production was significantly reduced in the mutant. Among them, we found a large variety of novel phased loci corresponding to protein-encoding genes or transposable elements. Interestingly, measurement of GFP expression revealed that post-transcriptional transgene silencing was reduced in rdr6.1 roots. Hence, this novel mis-sense mutation, affecting a highly conserved amino acid residue in plant RDR6s, may be an interesting tool both to analyse endogenous pha-siRNA functions and to improve transgene expression, at least in legume species.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
LEGUMES
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PHA-SIRNA
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RDR6
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RNA SILENCING
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SGS3
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SMALL RNAS
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TA-SIRNA
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A Medicago truncatula rdr6 allele impairs transgene silencing and endogenous phased siRNA production but not development
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-01-09T17:35:31Z
dc.journal.volume
12
dc.journal.number
9
dc.journal.pagination
1308-1318
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Bustos Sanmamed, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Institut Des Sciences Du Végétal; Francia
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Fil: Hudik, Elodie. Institut Des Sciences Du Végétal; Francia
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Fil: Laffont, Carole. Institut Des Sciences Du Végétal; Francia
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Fil: Reynes, Christelle. Molécules Thérapeutiques In Silico; Francia. Université Paris Diderot - Paris 7; Francia
dc.description.fil
Fil: Sallet, Erika. Laboratoire Des Interactions Plantes-microorganismes; Francia
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Fil: Wen, Jiangqi. The Samuel Roberts Noble Foundation; Estados Unidos
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Fil: Mysore, Kirankumar S.. The Samuel Roberts Noble Foundation; Estados Unidos
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Fil: Camproux, Anne Claude. Université Paris Diderot - Paris 7; Francia. Molécules Thérapeutiques In Silico; Francia
dc.description.fil
Fil: Hartmann, Caroline. Institut Des Sciences Du Végétal; Francia. Université Paris Diderot - Paris 7; Francia
dc.description.fil
Fil: Gouzy, Jérome. Laboratoire Des Interactions Plantes-microorganismes; Francia
dc.description.fil
Fil: Frugier, Florian. Institut Des Sciences Du Végétal; Francia
dc.description.fil
Fil: Crespi, Martin. Institut Des Sciences Du Végétal; Francia
dc.description.fil
Fil: Lelandais Brière, Christine. Institut Des Sciences Du Végétal; Francia. Université Paris Diderot - Paris 7; Francia
dc.journal.title
Plant Biotechnology Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/pbi.12230
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12230
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