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dc.contributor.author
Pérez de la Torre, Mariana Cecilia
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Fernández, Paula
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Greppi, Julián Alejandro
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Coviella, María Andrea
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Fernández, Martín Nicolás
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Astigueta, Francisco Horacio
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Mata, Diego Alejandro
dc.contributor.author
Trupkin, Santiago Ariel
dc.date.available
2020-01-09T20:50:43Z
dc.date.issued
2018-04
dc.identifier.citation
Pérez de la Torre, Mariana Cecilia; Fernández, Paula; Greppi, Julián Alejandro; Coviella, María Andrea; Fernández, Martín Nicolás; et al.; Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits; Elsevier Science; Scientia Horticulturae; 234; 4-2018; 300-311
dc.identifier.issn
0304-4238
dc.identifier.uri
http://hdl.handle.net/11336/94250
dc.description.abstract
Mecardonia constitutes an emergent crop in the floriculture market. In order to develop plants with improved compact growth by molecular breeding, we transformed Mecardonia cv. ‘Guarani Amarilla INTA’ using a wild-type strain of Agrobacterium rhizogenes. An advantage of using this approach is that generated plants are stable and considered non-GMO in several countries. Adventitious roots were produced by inoculating Mecardonia shoots with agropine ATCC15834 strain. Twenty independent root cultures regenerated spontaneously into shoots in hormone-free medium and presence of TL-DNA oncogenes were confirmed in their genomes. We selected four root inducing (Ri)-lines, designated GME, GCA, GVI and GIN, based on their different degree of compact growth habit. Under greenhouse conditions representative Ri-lines displayed reduced internode and shoot length, shoot and root biomass, aerial plant coverage, individual leaf area, flower width, pedicel length and the number of flowers per plant; and increased the number of nodes, axillary shoots, flower density and flower width relative to aerial plant coverage and mostly normal flowering when compared to non-transformed plants. To correlate phenotypical traits with gene expression, quantitative PCR analysis was performed. Ri-line GIN showed the highest rolA-D and ORF8, ORF13-14 gene expression which correlated with its super-dwarf phenotype, whereas the most weak Ri-phenotype observed in Ri-line GME showed no presence of rolA and ORF8 genes in plant genome. Expression of rolD and ORF13 correlated with reduced aerial plant coverage, shoot weight, shoot: root ratio and increased flower density and flower width relative to plant coverage, thus being considered of particular interest in Mecardonia breeding. Expression of ORF8 and rolA correlated with reduced aerial plant coverage, pedicel length, the total number of flowers per plant and increased flower width relative to the aerial plant coverage. Moreover, ORF8 and ORF13 may have a more prominent role in plant development than previously assumed and assigned to rol-genes. Overall, a better-organized compact growth without affecting other traits could be generated.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
MECARDONIA
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AGROBACTERIUM RHIZOGENES
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RI PLASMID
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COMPACT GROWTH
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ORNAMENTAL TRAITS
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ONCOGENE EXPRESSION
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NON-GMO
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Ciencias de las Plantas, Botánica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-12-20T22:54:03Z
dc.journal.volume
234
dc.journal.pagination
300-311
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Pérez de la Torre, Mariana Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
dc.description.fil
Fil: Fernández, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.description.fil
Fil: Greppi, Julián Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
dc.description.fil
Fil: Coviella, María Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
dc.description.fil
Fil: Fernández, Martín Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
dc.description.fil
Fil: Astigueta, Francisco Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.description.fil
Fil: Mata, Diego Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
dc.description.fil
Fil: Trupkin, Santiago Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
dc.journal.title
Scientia Horticulturae
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423818301225
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.scienta.2018.02.047
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