Artículo
Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta
Título:
Efeito do tempo de gestação em expressão de arnm de transportadores de ácidos graxos mRNA em placenta bovinos
Fecha de publicación:
01/2018
Editorial:
Univ Federal Uberlandia
Revista:
Bioscience Journal
ISSN:
1516-3725
e-ISSN:
1981-3163
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The objective of the study was to evaluate the effect of time of gestation on fatty acid transporter mRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4 per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles) and fetal (cotyledons) side. Once the placenta was removed, 1cm2 was dissected and, divided into caruncles and cotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data were analyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) with repeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereas animal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to a greater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. On the second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similar concentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were not different (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetal development.
Palabras clave:
Fatty acid binding protein
,
Fetal programming
,
Fatty acids.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - MAR DEL PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MAR DEL PLATA
Articulos(IGEVET)
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Articulos de INST.DE GENETICA VET ING FERNANDO NOEL DULOUT
Citación
Desantadina, Ramiro; Quintana, Silvina; Recavarren, Mariana Ines; Relling, Alejandro Enrique; Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta; Univ Federal Uberlandia; Bioscience Journal; 34; 1; 1-2018; 180-185
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