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Artículo

Neutralization of Junín virus by single domain antibodies targeted against the nucleoprotein

Linero, Florencia NataliaIcon ; Sepúlveda, Claudia SoledadIcon ; Christopoulou, Ioanna; Hulpiau, Paco; Scolaro, Luis AlbertoIcon ; Saelens, Xavier
Fecha de publicación: 12/2018
Editorial: Nature Publishing Group
Revista: Scientific Reports
ISSN: 2045-2322
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Virología

Resumen

The syndrome viral haemorrhagic fever (VHF) designates a broad range of diseases that are caused by different viruses including members of the family Arenaviridae. Prophylaxis for Argentine Haemorrhagic Fever (AHF), caused by the arenavirus Junín (JUNV), has been achieved by the use of a live attenuated vaccine, named Candid#1. The standard treatment of AHF is transfusion of convalescent human plasma. Our aim was to develop an alternative and safer treatment for AHF based on the use of virus-neutralizing single domain antibodies (VHHs). We describe the first reported VHHs directed against an arenavirus. These VHHs could neutralize Candid#1 by altering virion binding/fusion. Surprisingly, the neutralizing VHHs appeared to be specific for the viral nucleoprotein (N) that is not known to be involved in arenavirus entry. Candid#1 VHH-escape viruses had acquired a predicted N-glycosylation site in the surface glycoprotein GP1 that is present in highly pathogenic JUNV strains. Accordingly, the Candid#1-neutralizing VHHs could not neutralize pathogenic JUNV strains, but they could still bind to cells infected with a pathogenic strain or the escape mutant viruses. These results show that the attenuated strains of JUNV can be potently neutralized by nucleoprotein-specific VHHs.
Palabras clave: VHHs , Junín , Arenavirus , Neutralizing
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/94016
URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-6981-4_28
DOI: http://dx.doi.org/10.1038/s41598-018-29508-1
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Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Linero, Florencia Natalia; Sepúlveda, Claudia Soledad; Christopoulou, Ioanna; Hulpiau, Paco; Scolaro, Luis Alberto; et al.; Neutralization of Junín virus by single domain antibodies targeted against the nucleoprotein; Nature Publishing Group; Scientific Reports; 8; 11451; 12-2018; 1-17
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