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dc.contributor.author
Rius, Sebastian Pablo
dc.contributor.author
Casati, Paula
dc.contributor.author
Iglesias, Alberto Alvaro
dc.contributor.author
Gomez Casati, Diego Fabian
dc.date.available
2020-01-08T19:56:30Z
dc.date.issued
2006-08
dc.identifier.citation
Rius, Sebastian Pablo; Casati, Paula; Iglesias, Alberto Alvaro; Gomez Casati, Diego Fabian; Characterization of an Arabidopsis thaliana mutant lacking a cytosolic non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase; Springer; Plant Molecular Biology; 61; 6; 8-2006; 945-957
dc.identifier.issn
0167-4412
dc.identifier.uri
http://hdl.handle.net/11336/93996
dc.description.abstract
Non-phosphorylating glyceraldehyde- 3-phosphate dehydrogenase (NP-GAPDH) is a conserved cytosolic protein found in higher plants. In photosynthetic cells, the enzyme is involved in a shuttle transfer mechanism to export NADPH from the chloroplast to the cytosol. To investigate the role of this enzyme in plant tissues, we characterized a mutant from Arabidopsis thaliana having an insertion at the NP-GAPDH gene locus. The homozygous mutant was determined to be null respect to NP-GAPDH, as it exhibited undetectable levels of both transcription of NP-GAPDH mRNA, protein expression and enzyme activity. Transcriptome analysis demonstrated that the insertion mutant plant shows altered expression of several enzymes involved in carbohydrate metabolism. Significantly, cytosolic phosphorylating (NAD-dependent) glyceraldehyde-3-phosphate dehydrogenase mRNA levels are induced in the mutant, which correlates with an increase in enzyme activity. mRNA levels and enzymatic activity of glucose-6-phosphate dehydrogenase were also elevated, correlating with an increase in NADPH concentration. Moreover, increased ROS levels were measured in the mutant plants. Down-regulation of several glycolytic and photosynthetic genes suggests that NP-GAPDH is important for the efficiency of both metabolic processes. The results presented demonstrate that NP-GAPDH has a relevant role in plant growth and development.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ARABIDOPSIS
dc.subject
GLYCERALDEHYDES-3-P DEHYDROGENASE
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PLANT GLYCOLYSIS
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Characterization of an Arabidopsis thaliana mutant lacking a cytosolic non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-09-27T14:16:05Z
dc.identifier.eissn
1573-5028
dc.journal.volume
61
dc.journal.number
6
dc.journal.pagination
945-957
dc.journal.pais
Alemania
dc.description.fil
Fil: Rius, Sebastian Pablo.
dc.description.fil
Fil: Casati, P..
dc.description.fil
Fil: Iglesias, Alberto Alvaro.
dc.description.fil
Fil: Gomez Casati, Diego Fabian.
dc.journal.title
Plant Molecular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11103-006-0060-5
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11103-006-0060-5
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