Artículo
Trophoblast VIP deficiency entails immune homeostasis loss and adverse pregnancy outcome in mice
Hauk, Vanesa Cintia
; Vota, Daiana Marina
; Gallino, Lucila
; Calo, Guillermina
; Paparini, Daniel Esteban
; Merech, Fátima Isabel
; Ochoa, Federico Claudio; Zotta, Elsa; Ramhorst, Rosanna Elizabeth
; Waschek, James; Perez Leiros, Claudia
Fecha de publicación:
09/2018
Editorial:
Federation of American Societies for Experimental Biology
Revista:
FASEB Journal
ISSN:
0892-6638
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Immune homeostasis maintenance throughout pregnancy is critical for normal fetal development. Trophoblast cells differentiate into an invasive phenotype and contribute to the transformation of maternal arteries and the functional shaping of decidual leukocyte populations. Insufficient trophoblast invasion, inadequate vascular remodeling, and a loss of immunologic homeostasis are associated with pregnancy complications, such as preeclampsia and intrauterine growth restriction. Vasoactive intestinal peptide (VIP) is a pleiotropic neuropeptide synthetized in trophoblasts at the maternal–placental interface. It regulates the function of trophoblast cells and their interaction with decidual leukocytes. By means of a murine model of pregnancy in normal maternal background with VIP-deficient trophoblast cells, here we demonstrate that trophoblast VIP is critical for trophoblast function: VIP gene haploinsufficiency results in lower matrix metalloproteinase 9 expression, and reduced migration and invasion capacities. A reduced number of regulatory T cells at the implantation sites along with a lower expression of proangiogenic and antiinflammatory markers were also observed. Findings detected in the implantation sites at early stages were followed by an abnormal placental structure and lower fetal weight. This effect was overcome by VIP treatment of the early pregnant mice. Our results support the relevance of trophoblast-synthesized VIP as a critical factor in vivo for trophoblast-cell function and immune homeostasis maintenance in mouse pregnancy.
Palabras clave:
EARLY PREGNANCY
,
IMMUNOREGULATION
,
TROPHOBLAST FUNCTION
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Colecciones
Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Hauk, Vanesa Cintia; Vota, Daiana Marina; Gallino, Lucila; Calo, Guillermina; Paparini, Daniel Esteban; et al.; Trophoblast VIP deficiency entails immune homeostasis loss and adverse pregnancy outcome in mice; Federation of American Societies for Experimental Biology; FASEB Journal; 33; 2; 9-2018; 1801-1810
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