Artículo
Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities
Caminata Landriel, Soledad; Castillo, Julieta de Las Mercedes; Taboga, Oscar Alberto
; Ferrarotti, Susana Alicia; Gottlieb, Alexandra Marina
; Costa, Hernán
Fecha de publicación:
12/2018
Editorial:
Academia Brasileira de Ciencias
Revista:
Anais da Academia Brasileira de Ciencias
ISSN:
0001-3765
e-ISSN:
1678-2690
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β-and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.
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Articulos(INEDES)
Articulos de INSTITUTO DE ECOLOGIA Y DESARROLLO SUSTENTABLE
Articulos de INSTITUTO DE ECOLOGIA Y DESARROLLO SUSTENTABLE
Citación
Caminata Landriel, Soledad; Castillo, Julieta de Las Mercedes; Taboga, Oscar Alberto; Ferrarotti, Susana Alicia; Gottlieb, Alexandra Marina; et al.; Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 91; 3; 12-2018; 1-11; e20180568
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