A fast chromatographic method for determination of daidzein and genistein in spiked water river samples using multivariate curve resolution

Abstract

This work reports the development of a fast chromatography methodology for quantitation of two phytoestrogens: daidzein (DAI) and genistein (GEN), in river water samples. The proposed method is based on High performance liquid chromatography-diode array detection (HPLC-DAD) data, and multivariate curve resolution-alternative least square (MCR-ALS) second-order calibration. Initially, the method was evaluated analysing a synthetic validation set, prepared based on a Taguchi design. Subsequently, the method was applied to predict the concentration of the phytoestrogens in river water samples, previously pre-processed by solid phase extraction (SPE). By implementation of the present methodology, a 50 % of reduction in operation time was achieved (from 7.00 to 3.25 min) when compared with previous work in literature. Precision were achieved even in the presence non modelled constituents and strong background. Thus, it has shown that the proposed method to be abecome in a rapid and robust alternative for the quantitation of studied phytoestrogens.