Artículo
Penicillamine determination using a tyrosinase micro-rotating biosensor
Torriero, Angel Alberto Jesus
; Salinas, Eloy; Marchevsky, Eduardo Jorge
; Raba, Julio
; Silber, Juana J.
Fecha de publicación:
11/2006
Editorial:
Elsevier Science
Revista:
Analytica Chimica Acta
ISSN:
0003-2670
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on glassy carbon electrode surface at -150 mV versus Ag/AgCl/NaCl 3 M. Thus, when penicillamine (PA) was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used for sensitive determination of PA in drug and human synthetic serum samples. A linear range of 0.02-80 μM (r = 0.999) was obtained for amperometric determination of PA in buffered pH 7.0 solutions (0.1 M phosphate buffer). The biosensor has a reasonable reproducibility (R.S.D. < 4.0%) and a very stable amperometric response toward this compound (more than 1 month).
Palabras clave:
4-TERT-BUTYLCATECHOL
,
BIOSENSOR
,
GLASSY CARBON
,
PENICILLAMINE
,
TYROSINASE
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Torriero, Angel Alberto Jesus; Salinas, Eloy; Marchevsky, Eduardo Jorge; Raba, Julio; Silber, Juana J.; Penicillamine determination using a tyrosinase micro-rotating biosensor; Elsevier Science; Analytica Chimica Acta; 580; 2; 11-2006; 136-142
Compartir
Altmétricas